文献类型：期刊文献

英文题名：Rapid Identification of the Receptor-Binding Specificity of InfluenzaA Viruses by Fluorogenic Glycofoldamers

作者：He, Xiao-Peng[1,2];Zeng, Ya-Li[1,2];Tang, Xin-Ying[3];Li, Na[4];Zhou, Dong-Ming[3];Chen, Guo-Rong[1,2];Tian, He[1,2]

机构：[1]East China Univ Sci & Technol, Key Lab Adv Mat, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Chem & Mol Engn, Inst Fine Chem, 130 Meilong Rd, Shanghai 200237, Peoples R China;[3]Chinese Acad Sci, Inst Pasteur Shanghai, Vaccine Res Ctr, Key Lab Mol Virol & Immunol, Shanghai 200031, Peoples R China;[4]Chinese Acad Sci, Shanghai Inst Biol Sci, Natl Ctr Prot Sci Shanghai, Shanghai 200031, Peoples R China

年份：2016

卷号：55

期号：45

起止页码：13995

外文期刊名：ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

收录：;EI(收录号：20163302719723);WOS:【SCI-EXPANDED(收录号:WOS:000387016200013)】；

基金：This research was supported by the 973 project (2013CB733700), the National Natural Science Foundation of China (21572058, 21576088, and 31200617), the Knowledge Innovation Program (Y014P31503), the 100 Talent Program (Y316P11503) from the Chinese Academy of Sciences (to D.Z.), and the Shanghai Rising-Star Program (16QA1401400 to X.-P.H.). We also thank Dr. Chen Ling for providing virus isolates.

语种：英文

外文关键词：fluorescence spectroscopy; foldamers; glycans; high-throughput screening; influenza

摘要：The re-emergence of influenza raises a global concern that viral pandemics can unpredictably occur. However, effective approaches that can probe the infection risk of influenza viruses for humans are rare. In this work, we develop a glycofoldamer that can rapidly identify the glycan-receptor specificity of influenza viruses in a high-throughput manner. The coupling of glycan receptors that can be recognized by hemagglutinin (a surface protein on the virion capsid of influenza) to a fluorogenic-dye foldamer produces the glycofoldamers with minimal fluorescence in aqueous solution. After interaction with human-infecting virus strains for only five minutes, the fluorescence intensity of the glycofoldamer is remarkably enhanced with a blue-shifted emission peak. The probes have also proven effective for the rapid identification of 1)the human- or bird-infecting properties of influenza viruses in a high-throughput manner and 2)the receptor-specificity switch of a virus strain by mutations.