文献类型：期刊文献

英文题名：DeoR regulates lincomycin production in Streptomyces lincolnensis

作者：Zou, Jingyun[1,2];Mao, Yue[1,2];Hou, Bingbing[1,2];Kang, Yajing[1,2];Wang, Ruida[1,2];Wu, Haizhen[1,2];Ye, Jiang[1,2];Zhang, Huizhan[1,2]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Dept Appl Biol, Shanghai 200237, Peoples R China

年份：2023

卷号：39

期号：12

外文期刊名：WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY

收录：;EI(收录号：20234114876916);WOS:【SCI-EXPANDED(收录号:WOS:001081834900001)】；

基金：This work was supported by Ministry of Science and Technology (MOST) of China (2021YFC2100600) and the National Natural Science Foundation of China (NSFC) (31900059)

语种：英文

外文关键词：Streptomyces lincolnensis; Lincomycin; DeoR family; Transcriptional regulation

摘要：Regulators belonging to the DeoR family are widely distributed among the bacteria. Few studies have reported that DeoR family proteins regulate secondary metabolism of Streptomyces. This study explored the function of DeoR (SLINC_8027) in Streptomyces lincolnensis. Deletion of deoR in NRRL 2936 led to an increase in cell growth. The lincomycin production of the deoR deleted strain Delta deoR was 3.4-fold higher than that of the wild strain. This trait can be recovered to a certain extent in the deoR complemented strain Delta deoR::pdeoR. According to qRT-PCR analysis, DeoR inhibited the transcription of all detectable genes in the lincomycin biosynthesis cluster and repressed the expression of glnR, bldD, and SLCG_Lrp, which encode regulators outside the cluster. DeoR also inhibited the transcription of itself, as revealed by the XylE reporter. Furthermore, we demonstrated that DeoR bound directly to the promoter region of deoR, lmbA, lmbC-D, lmbJ-K, lmrA, lmrC, glnR, and SLCG_Lrp, by recognizing the 5'-CGATCR-3' motif. This study found that versatile regulatory factor DeoR negatively regulates lincomycin biosynthesis and cellular growth in S. lincolnensis, which expanded the regulatory network of lincomycin biosynthesis.