文献类型：期刊文献

中文题名：MPure-12全自动核酸纯化仪与Chelex-100法的比较

英文题名：Comparison of MPure-12 Automatic Nucleic Acid Purification and Chelex-100 Method

作者：盛翔[1,2];李敏[2,3];王亚丽[2,4];陈玉玲[2,5];林源[2];赵珍敏[2];阙庭志[2]

机构：[1]苏州大学医学部法医学系,江苏苏州215123;[2]司法部司法鉴定科学技术研究所上海市法医学重点实验室上海市司法鉴定专业技术服务平台,上海200063;[3]温州医科大学基础医学院,浙江温州325000;[4]内蒙古医科大学基础医学院,内蒙古呼和浩特010020;[5]华东理工大学生物工程学院生物反应器工程国家重点实验室,上海200237

年份：2017

卷号：33

期号：2

起止页码：168

中文期刊名：法医学杂志

外文期刊名：Journal of Forensic Medicine

收录：CSTPCD;;Scopus;CSCD:【CSCD_E2017_2018】；PubMed;

基金：十三五国家重点研发计划项目(2016YFC0800703);中央级科研院所公益资助项目(GY2014G-4,GY2017D-2);上海市法医学重点实验室资助项目(17DZ2273200);上海市司法鉴定专业技术服务平台资助项目(16DZ2290900)

语种：中文

中文关键词：法医遗传学;提取法;自动化,实验室;MPure-12全自动核酸纯化仪;Chelex-100法

外文关键词：forensic genetics; extraction; automation, laboratory; MPure-12 automatic nucleic acid purification; Chelex-100 method

摘要：目的通过对DNA含量不同的血痕和多种类型生物学检材进行DNA提取和STR分型检测,探讨MPure-12全自动核酸纯化仪(MPure-12法)在DNA提取中的法医学应用价值。方法收集血痕、精斑、唾液等9种类型的生物学检材,应用MPure-12法和传统Chelex-100法提取DNA,经过PCR扩增和电泳,获取STR分型图谱。结果 MPure-12法对发根、口香糖、烟蒂、肌肉组织、唾液斑、血痕、精斑这些检材能够成功分型,唾液出现了个别等位基因丢失现象,接触拭子分型较差。Chelex-100法对血量为20μL、15μL、10μL、5μL、1μL制成的血痕均有完整分型结果,MPure-12法在血量为1μL时出现等位基因丢失现象。结论 MPure-12法适用于一定浓度血样的检验,而对于微量血样,Chelex-100法提取DNA的效果可能更优。仪器应用于DNA提取具有操作简单、快速、提取效率高,分型成功率高,减少人为污染等优势。
Objective To explore the forensic application value of MPure-12 automatic nucleic acid purification （MPure-12 Method） for DNA extraction by extracting and typing DNA from bloodstains and various kinds of biological samples with different DNA contents. Methods Nine types of biological sam-ples, such as bloodstains, semen stains, and saliva were collected. DNA were extracted using MPure-12 method and Chelex-100 method, followed by PCR amplification and electrophoresis for obtaining STR- profiles. Results The samples such as hair root, chutty, butt, muscular tissue, saliva stain, bloodstain and semen stain were typed successfully by MPure-12 method. Partial alleles were lacked in the samples of saliva, and the genotyping of contact swabs was unsatisfactory. Additional, all of the bloodstains （20μL, 15μL, 10μL, 5μL, 1μL） showed good typing results using Chelex-100 method. But the loss of alleles occurred in 1μL blood volume by MPure-12 method. Conclusion MPure-12 method is suitable for DNA extraction of a certain concentration blood samples. Chelex-100 method may be better for the extraction of trace blood samples. This instrument used in nucleic acid extraction has the advantages of simplicity of operator, rapidity, high extraction efficiency, high rate of reportable STR-profiles and lower man-made pollution.