文献类型：期刊文献

英文题名：Polyhedral Oligomeric Silsesquioxane-Incorporated Gelatin Hydrogel Promotes Angiogenesis during Vascularized Bone Regeneration

作者：Chen, Mingjiao[2];Zhang, Yuanhao[1];Zhang, Weian[1];Li, Jin[2]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]Shanghai Jiao Tong Univ, Peoples Hosp 9, Shanghai Key Lab Orbital Dis & Ocular Oncol, Dept Ophthalmol,Sch Med, Shanghai 200011, Peoples R China

年份：2020

卷号：12

期号：20

起止页码：22410

外文期刊名：ACS APPLIED MATERIALS & INTERFACES

收录：;EI(收录号：20202308793248);WOS:【SCI-EXPANDED(收录号:WOS:000537394100002)】；

基金：This work was supported by the National Key R&D Program of China (2018YFC1106100, 2018YFC1106101), Science and Technology Commission of Shanghai (17DZ2260100), National Natural Science Foundation of China (81870688), Shanghai Municipality Commission for Science and Technology (13DZ0500303), and Shanghai International Cooperation Program (19440710600). Dr. Ping Gu and Dr. Huifang Zhou helped with the literature and assisted in formatting the text. Dr. Xianqun Fan contributed to the conception and design of initial manuscript and the data analysis. Dr. Xianqun Fan also provided the foundation support, modified the structure of the revised manuscript, and helped to comment on the revised manuscript.

语种：英文

外文关键词：angiogenesis; bone regeneration; POSS; hydrogels; nanoparticles

摘要：Many approaches have been made toward the development of scaffolds with good biocompatibility and appreciable physicochemical properties to facilitate stem cell adhesion, osteogenic differentiation, and vascularization in tissue engineering. Nowadays, vascularization is a main bottleneck in tissue engineering strategies that is needed to be overcome and developed. Herein, we construct a series of polyhedral oligomeric silsesquioxane (POSS)-modified porous gelatin hydrogels with different POSS concentrations from 0 to 5 wt %, defined as X% POSS hydrogels (X = 0, 1, 2, 3, 4, 5) to support vascularized bone repair. The introduction of POSS into gelatin effectively promoted adhesive protein adsorption and integrin alpha 5 beta 1 expression, subsequently leading to enhanced adhesion of both rat bone marrow mesenchymal stem cells and human umbilical vein endothelial cells (HUVECs). In vitro experiments further demonstrated that POSS-containing hybrid hydrogels more effectively support the angiogenic tube and network formation in HUVECs than the 0% POSS hydrogel. Besides, POSS-containing hybrid hydrogels showed desirable performance as a sustained release system of vascular endothelial growth factor (VEGF) and bone morphogenetic protein-2 (BMP-2), and they further accelerated vascular network establishment and the formation of a new bone in defect regions. When the hydrogels were implanted into critical-sized rat calvarial defects in vivo, the VEGF/BMP-2-coupled 3% POSS group gained a higher blood vessel volume in the bone defect regions (5.49 +/- 0.35 mm(3)) than the 3% POSS group (3.12 +/- 0.20 mm(3)) and the 0% POSS group (1.57 +/- 0.25 mm(3)), suggesting that the 3% POSS hydrogel with VEGF/BMP-2 would expedite vascularization. Based on these evaluations, our results indicated that the POSS-incorporated gelatin hydrogel would provide a promising bone graft scheme in potential clinical application of large bone defect repair.