文献类型：期刊文献

英文题名：Biomimetic de novo Construction of Hierarchically Aligned and Gradient-Mineralized Collagen for Tendon-Bone Integrated Regeneration

作者：Lei, Miao[1]; Luo, Hao[1]; Sun, Luyi[2]; Shi, Songsong[1]; Zhang, Zhuangri[1]; Yu, Chengxuan[2]; Gao, Han[2]; Chen, Jun[2]; Liu, Changsheng[1]; Qu, Xue[1,3]

机构：[1] Key Laboratory for Ultrafine Materials of Ministry of Education, Frontiers Science Center for Materiobiology and Dynamic Chemistry, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai, 200237, China; [2] Sports Medicine Institute of Fudan University, Department of Sports Medicine, Huashan Hospital, Fudan University, Shanghai, 200040, China; [3] Shanghai Frontier Science Research Base of Optogenetic Techniques for Cell Metabolism, East China University of Science and Technology, Shanghai, 200237, China

年份：2025

外文期刊名：SSRN

收录：EI(收录号：20250525871)

语种：英文

外文关键词：Biomimetics - Bone - Elasticity - Matrix algebra - Mineralogy - Minerals - Tissue regeneration

摘要：The tissues spanning from tendon to bone exhibit a highly specialized extracellular matrix (ECM) architecture, characterized by hierarchical collagen alignment and a gradient mineral composition, which together enable efficient force transfer and guide spatially organized cellular phenotypes. However, recapitulating such complex multi-scale organization and compositional gradients to achieve integrated soft-hard tissue remains challenging. Here, we report the de novo construction of biomimetic collagen–mineral matrices that mimic both the hierarchical organization and mineral gradient distribution of the native tendon-to-bone ECM. Through synergistic electroassembly and post-treatment, collagen molecules self-organized into aligned fibrillar matrices with multi-scale architecture, replicating tendon-side morphology while providing robust tensile mechanics. At the opposing end, intrafibrillar and interfibrillar minerals were spatially patterned to emulate the mineral gradient from tendon to bone. This structural and compositional continuum enables smooth mechanical transition across the soft-hard tissue interface and promotes region-specific regeneration of aligned tendon-like tissue, fibrocartilage and bone. In vivo studies in rabbit models confirm that these de novo constructed matrices support histological reconstruction of multiple tissues from tendon to bone at the rotator cuff, and significantly improve functional recovery. This work presents a bottom-up biomimetic strategy for engineering multiscale collagen-based scaffolds and demonstrates the therapeutic potential of de novo constructed matrices for multiple tissue regeneration. ? 2025, The Authors. All rights reserved.