文献类型：期刊文献

英文题名：A PDMS/paper/glass hybrid microfluidic biochip integrated with aptamer-functionalized graphene oxide nano-biosensors for one-step multiplexed pathogen detection

作者：Zuo, Peng[1];Li, XiuJun[1,2];Dominguez, Delfina C.[3];Ye, Bang-Ce[2]

机构：[1]Univ Texas El Paso, Dept Chem, El Paso, TX 79968 USA;[2]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[3]Univ Texas El Paso, Coll Hlth Sci, El Paso, TX 79968 USA

年份：2013

卷号：13

期号：19

起止页码：3921

外文期刊名：LAB ON A CHIP

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000323835700017)】；

基金：Research reported in this publication was supported by the National Institute Of General Medical Sciences of the National Institutes of Health under Award Number SC2GM105584. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. Financial support from the Interdisciplinary Research (IDR level 2) Program at the University of Texas at El Paso, University of Texas (UT) System for the STARS award, and State Key Laboratory of Bioreactor Engineering of China for the Open Project Fund are also gratefully acknowledged.

语种：英文

摘要：Infectious pathogens often cause serious public health concerns throughout the world. There is an increasing demand for simple, rapid and sensitive approaches for multiplexed pathogen detection. In this paper we have developed a polydimethylsiloxane (PDMS)/paper/glass hybrid microfluidic system integrated with aptamer-functionalized graphene oxide (GO) nano-biosensors for simple, one-step, multiplexed pathogen detection. The paper substrate used in this hybrid microfluidic system facilitated the integration of aptamer biosensors on the microfluidic biochip, and avoided complicated surface treatment and aptamer probe immobilization in a PDMS or glass-only microfluidic system. Lactobacillus acidophilus was used as a bacterium model to develop the microfluidic platform with a detection limit of 11.0 cfu mL(-1). We have also successfully extended this method to the simultaneous detection of two infectious pathogens - Staphylococcus aureus and Salmonella enterica. This method is simple and fast. The one-step 'turn on' pathogen assay in a ready-to-use microfluidic device only takes similar to 10 min to complete on the biochip. Furthermore, this microfluidic device has great potential in rapid detection of a wide variety of different other bacterial and viral pathogens.