文献类型：期刊文献

英文题名：Long-term capsaicin intake and gut inflammation: microbial alterations, metabolic mechanisms, and intervention effects

作者：Zhu, Liangliang[1];Chen, Yijia[1];Jin, Jiayang[1,4];Zhao, Mengyao[1,3];Ran, Jingfang[1];Yin, Hao[2];Ji, Xiaoguo[1,3];Zhao, Liming[1,2,4]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shanghai Chang Zheng Hosp, Organ Transplant Ctr, Shanghai 200003, Peoples R China;[3]East China Univ Sci & Technol, Shanghai Frontiers Sci Ctr Optogenet Tech Cell Met, Sch Pharm, 130 Mei Long Rd, Shanghai 200237, Peoples R China;[4]Shanghai Collaborat Innovat Ctr Biomfg Technol SCI, Shanghai 200237, Peoples R China

年份：2026

卷号：231

外文期刊名：FOOD RESEARCH INTERNATIONAL

收录：;EI(收录号：20260920177365);WOS:【SCI-EXPANDED(收录号:WOS:001706529800001)】；

基金：This work was supported by The Young Scientists Fund of the National Natural Science Foundation of China (32302102) , Natural Science Foundation of Shanghai (23ZR1415400) and Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism (Shanghai Municipal Education Commission) .

语种：英文

外文关键词：Capsaicin; Metabolomics; Vanillate decarboxylase; Caco-2 cell; Human microbiota-associated animal

摘要：The effects of capsaicin consumption on gut health and its mechanisms remain uncharacterized. This study integrated human cohort and colonic simulation fermentation found that long-term capsaicin intake induced gut dysbiosis, characterized by an increase in Klebsiella and a decrease in Lactobacillus, which correlated significantly with gut inflammation and the dysregulation of key metabolites (including bile acids, short-chain fatty acids derivatives and steroids). Capsaicin metabolites, such as dihydrocapsaicin, ferulic acid, and veratric acid were identified. The study also enriched and identified capsaicin-utilizing gut microbes, with Escherichia and Klebsiella being predominant. Through genomic homology analysis, machine learning prediction, and molecular docking simulations, vanillate decarboxylase homologous protein potentially involved in capsaicin metabolism was identified in strains including Escherichia coli, Klebsiella aerogenes, and Enterococcus faecalis and its expression was positively correlated with capsaicin metabolic efficiency. In cell and human microbiota-associated animals, Lactobacillus plantarum and Enterococcus faecalis significantly alleviated capsaicin-induced gut inflammation and repaired the intestinal barrier function by directly degrading capsaicin. This research elucidated the mechanism by which long-term capsaicin altered gut microbiota and its metabolites to induce inflammation, providing a foundation for managing gut health of individuals with chili habit.