文献类型：期刊文献

英文题名：Asymmetric Amination of Secondary Alcohols by using a Redox-Neutral Two-Enzyme Cascade

作者：Chen, Fei-Fei[1];Liu, You-Yan[2,3];Zheng, Gao-Wei[1];Xu, Jian-He[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai Collaborat Innovat Ctr Biomfg, Shanghai 200237, Peoples R China;[2]Guangxi Univ, Sch Chem & Chem Engn, Nanning 530004, Guangxi, Peoples R China;[3]Guangxi Acad Sci, Guangxi Key Lab Biorefinery, Nanning 530003, Guangxi, Peoples R China

年份：2015

卷号：7

期号：23

起止页码：3838

外文期刊名：CHEMCATCHEM

收录：;EI(收录号：20160401843734);WOS:【SCI-EXPANDED(收录号:WOS:000366837000006)】；

基金：This work was financially supported by the National Natural Science Foundation of China (No. 21472045, 21276082, 31200050, and 21536004), Ministry of Science and Technology, P. R. China (Nos. 2011CB710800), the Fundamental Research Funds for the Central Universities (22A201514043), and Guangxi Key Laboratory of Biorefinery.

语种：英文

外文关键词：amine dehydrogenase; asymmetric catalysis; biotransformations; cascade reaction; chiral amines

摘要：Multienzyme cascade approaches for the synthesis of optically pure molecules from simple achiral compounds are desired. Herein, a cofactor self-sufficient cascade protocol for the asymmetric amination of racemic secondary alcohols to the corresponding chiral amines was successfully constructed by employing an alcohol dehydrogenase and a newly developed amine dehydrogenase. The compatibility and the identical cofactor dependence of the two enzymes led to an ingenious in situ cofactor recycling system in the one-pot synthesis. The artificial redox-neutral cascade process allowed the transformation of racemic secondary alcohols into enantiopure amines with considerable conversions (up to 94%) and >99% enantiomeric excess at the expense of only ammonia; this method thus represents a concise and efficient route for the asymmetric synthesis of chiral amines.