文献类型：期刊文献

中文题名：发酵摇瓶测氧电极FCY-Ⅲ型的研制及其应用

英文题名：The Study of DO Electrode for Shake Flask Culture and Applications

作者：李友荣[1];储炬[1]

机构：[1]华东化工学院生化工程系

年份：1991

卷号：7

期号：1

起止页码：95

中文期刊名：生物工程学报

外文期刊名：Chinese Journal of Biotechnology

收录：CSTPCD;;Scopus;北大核心:【北大核心1992】；CSCD:【CSCD2011_2012】；PubMed;

语种：中文

中文关键词：发酵摇瓶;菌株筛选;测氧电极

外文关键词：Dissolved oxygen electrode;;spiramycin fermentation;;penicilli fermentation

摘要：筛选高产变株的最常用的行之有效的手段是上发酵摇瓶,而普通的发酵摇瓶往往只能测得其初始和最终的参数,要想知道发酵过程中的参数变化情况是比较困难的,且取样测定会明显改变发酵液体积。况且取样来测定溶氧是不可靠的。为此。
Shake flasks have been frequently employed for the screening ofhigh productivity strains. However, DO, which is one of the mostimportant parameters, is quite difficult to be monitored in shake flask.culture. A new model of DO probe specially designed for shake flaskculture is of galvanic type. The interior electrode body, which is com-posed of silver (as cathode) and lead (as anode), is inserted in a glassceramic housing. The latter is served as an electrolyte reservior. Theglass ceramic housing can be inserted in a standard ground glass sidetube of 750 ml shake flask. The electrode has been applied to spira-mycin, penicillin fermentation and arthrobacter culture. The informationmonitored by the DO probe proved to be very useful in evaluating theculture process.