文献类型：期刊文献

英文题名：Structure elucidation and immunological activity of a novel glycopeptide from mannatide

作者：Shen, Yanhong[1];Wu, Mengqi[1];Xu, Zhizhen[1];Wan, Yanfei[2];Zhang, Li[2];Zhang, Wenqing[1];Xia, Wei[1]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Chengdu Leer Pharmaceut Co Ltd, Chengdu 610083, Peoples R China

年份：2017

卷号：99

起止页码：112

外文期刊名：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

收录：;EI(收录号：20240815591425);WOS:【SCI-EXPANDED(收录号:WOS:000400034100014)】；

基金：This project was supported by Chengdu Leer Pharmaceutical Co., Ltd. of Chengdu, China.

语种：英文

外文关键词：Mannatide; Polysaccharide; Structure; Immunological activity

摘要：A water-soluble glycopeptide, designated as MTW, was isolated and purified from crude mannatide by DEAE-Sepharose. MTW showed one symmetrical peak on HPGPC with an average molecular weight of 2.95 x 10(4) Da. MTW contained 16 kinds of amino acids and the total amino acid content was 0.95%. The structure of polysaccharide moiety of MTW was elucidated based on monosaccharide composition, methylation analysis, partial acid hydrolysis, IR and 1D/2D NMR spectroscopy. The results showed that MTW was a homogeneous glycopeptide including mannose and glucose with a molar ratio of 2:1. The polysaccharide moiety of MTW had a backbone of (1 -> 6)-alpha-D-Man p residues, which highly branched at O-2 position of (1 -> 2,6)-alpha-D-Man p residues. The side chains were mainly composed of (1 ->)-alpha-D-Man p, (1 -> 2)-alpha-D-Man p, (1 -> 4)-alpha-D-Glc p, (1 -> 4,6)-alpha-D-Glc p residues. The backbone of the polysaccharide moiety of MTW was the same as MT2-A (Li et al. [1]), which was the main component of mannatide, but the side chains of MTW were somewhat different from that of MT2-A. Complement-fixation test indicated that MTW had the same beneficial effect on immunological activity as MT2-A. (C) 2017 Elsevier B.V. All rights reserved.