文献类型：期刊文献

英文题名：Engineering thermophilic Geobacillus thermoglucosidasius for riboflavin production

作者：Yang, Zhiheng[1];Sun, Qingqing[2];Tan, Gaoyi[1];Zhang, Quanwei[2];Wang, Zhengduo[1];Li, Chuan[1];Qi, Fengxian[2];Wang, Weishan[1,2];Zhang, Lixin[1];Li, Zilong[2]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing 100101, Peoples R China

年份：2021

卷号：14

期号：2

起止页码：363

外文期刊名：MICROBIAL BIOTECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000625633600003)】；

语种：英文

摘要：The potential advantages for fermentation production of chemicals at high temperatures are attractive, such as promoting the rate of biochemical reactions, reducing the risk of contamination and the energy consumption for fermenter cooling. In this work, we de novo engineered the thermophile Geobacillus thermoglucosidasius to produce riboflavin, since this bacterium can ferment diverse carbohydrates at an optimal temperature of 60 degrees C with a high growth rate. We first introduced a heterogeneous riboflavin biosynthetic gene cluster and enabled the strain to produce detectable riboflavin (28.7 mg l(-1)). Then, with the aid of an improved gene replacement method, we preformed metabolic engineering in this strain, including replacement of ribC(Gtg) with a mutant allele to weaken the consumption of riboflavin, manipulation of purine pathway to enhance precursor supply, deletion of ccpN(Gtg) to tune central carbon catabolism towards riboflavin production and elimination of the lactate dehydrogenase gene to block the dominating product lactic acid. Finally, the engineered strain could produce riboflavin with the titre of 1034.5 mg l(-1) after 12-h fermentation in a mineral salt medium, indicating G. thermoglucosidasius is a promising host to develop high-temperature cell factory of riboflavin production. This is the first demonstration of riboflavin production in thermophilic bacteria at an elevated temperature.