文献类型：期刊文献

英文题名：Detection of Carbapenemase-Producing Organisms with a Carbapenem-Based Fluorogenic Probe

作者：Mao, Wuyu[1];Xia, Lingying[1];Xie, Hexin[1]

机构：[1]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab New Drug Design, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2017

卷号：56

期号：16

起止页码：4468

外文期刊名：ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

收录：;EI(收录号：20171303498075);WOS:【SCI-EXPANDED(收录号:WOS:000398154000009)】；

基金：This study is financially supported by the National Natural Science Foundation of China (21472048), the Fundamental Research Funds for the Central Universities, the "Thousand Plan" Youth Program, and the East China University of Science & Technology. We thank Prof. Jianghong Rao at Stanford University for kindly providing beta-lactamase plasmids.

语种：英文

外文关键词：antibiotic resistance; carbapenemases; fluorescent probes; beta-lactamases

摘要：Antibiotic resistance has become a major challenge to public health worldwide. This crisis is further aggravated by the increasing emergence of bacterial resistance to carbapenems, typically considered as the antibiotics of last resort, which is mainly due to the production of carbapenem-hydrolyzing carbapenemases in bacteria. Herein, the carbapenem-based fluorogenic probe CB-1 with an unprecedented enamine-BODIPY switch is developed for the detection of carbapenemase activity. This reagent is remarkably specific towards carbapenemases over other prevalent beta-lactamases. Furthermore, the efficient imaging of live clinically important carbapenemase-producing organisms (CPOs) with CB-1 demonstrates its potential for the rapid detection of antibiotic resistance and timely diagnosis of CPO infections.