文献类型：期刊文献

英文题名：In vitro anti-tumor activity of isorhamnetin isolated from Hippophae rhamnoides L. against BEL-7402 cells

作者：Teng, Bao-song; Lu, Yan-Hua; Wang, Zheng-Tao; Tao, Xin-Yi; Wei, Dong-Zhi

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, New World Inst Biotechnol, Box 311 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Shanghai Univ TCM, Inst Chinese Mat Med, Shanghai 201203, Peoples R China

年份：2006

卷号：54

期号：3

起止页码：186

外文期刊名：PHARMACOLOGICAL RESEARCH

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000240376600004)】；

语种：英文

外文关键词：flavonoids; tumor; isorhamnetin; proliferation; apoptosis

摘要：Isorhamnetin, a flavonol aglycone, isolated from the traditional Chinese medicine Hippophae rhamnoides L., was investigated in its cytotoxicity and its influence on human hepatocellular carcinoma cells (BEL-7402). The cytotoxic effects of isorhamnetin showed dose- and time-dependency against BEL-7402 cells, with IC50 equal to 74.4 +/- 1.13 mu g ml(-1) after treatment with isorhamnetin for 72 h. Cytotoxicity of the flavonols on tumor cells depends on cellular accumulation of the drugs. The amount of isorhamnetin accumulated in BEL-7402 cells was assayed by high-performance liquid chromatography (HPLC) and showed that isorhamnetin could permeate the cell membrane into the cell. Staining with Hoechst 33258 showed fragmentation and condensation of chromatin in the cell treated with 50 mu g ml-1 isorhamnetin for 48 h. Flow cytometric analysis was performed to determine hypodiploid cells. The results of flow cytometry assay indicated that the percentage of hypodiploid BEL-7402 cells were 13.77 +/- 1.05% after 48 h treatment with 50 mu g ml(-1) isorhamnetin. The treatment resulted in the appearance of a hypodiploid peak (sub-GO/Gl peak), probably due to the presence of cells in apoptosis and apoptotic bodies with DNA content less than 2n. To our knowledge, this is the first report against human hepatocellular carcinoma cells (BEL-7402) of isorhamnetin. (c) 2006 Elsevier Ltd. All rights reserved.