文献类型：期刊文献

中文题名：Comparative omics directed gene discovery and rewiring for normal temperature-adaptive red pigment synthesis by polar psychrotrophic fungus Geomyces sp.WNF-15A

作者：Haoyu Long[1];Jiawei Zhou[1];Yanna Ren[1];Jian Lu[1];Nengfei Wang[2];Haifeng Liu[3];Xiangshan Zhou[4];Menghao Cai[1,5]

机构：[1]State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,Shanghai,200237,China;[2]School of Chemistry&Chemical Engineering,Linyi University,Linyi,276005,China;[3]China Resources Angde Biotech Pharma Co.,Ltd.,78 E-jiao Street,Liaocheng,China;[4]China Resources Biopharmaceutical Co.,Ltd.,1301-84 Sightseeing Road,Shenzhen,China;[5]Shanghai Collaborative Innovation Center for Biomanufacturing,Shanghai,200237,China

年份：2024

卷号：9

期号：4

起止页码：842

中文期刊名：Synthetic and Systems Biotechnology

外文期刊名：合成和系统生物技术(英文)

收录：CSCD:【CSCD2023_2024】；

基金：supported by the National Natural Science Foundation of China(42176238);the 111 Project of China(B18022).

语种：英文

中文关键词：Polar fungi;Red pigment;Cold-adaptive;Comparative omics;Food additives

摘要：The Antarctic fungus Geomyces sp.WNF-15A can produce high-quality red pigments(AGRP)with good prospects for the use in food and cosmetic area.However,efficient AGRP synthesis relies on low-temperature and thus limits its industrial development.Here genome sequencing and comparative analysis were performed on the wild-type versus to four mutants derived from natural mutagenesis and transposon insertion mutation.Eleven mutated genes were identified from 2309 SNPs and 256 Indels.A CRISPR-Cas9 gene-editing system was established for functional analysis of these genes.Deficiency of scaffold1.t692 and scaffold2.t704 with unknown functions highly improved AGRP synthesis at all tested temperatures.Of note,the two mutants produced comparable levels of AGRP at 20℃to the wild-type at 14℃.They also broke the normal-temperature limitation and effectively synthesized AGRP at 25℃.Comparative metabolomic analysis revealed that deficiency of scaffold1.t692 improved AGRP synthesis by regulation of global metabolic pathways especially downregulation of the competitive pathways.Knockout of key genes responsible for the differential metabolites confirmed the metabolomic results.This study shows new clues for cold-adaptive regulatory mechanism of polar fungi.It also provides references for exploitation and utilization of psychrotrophic fungal resources.