文献类型：期刊文献

中文题名：Structural analysis of recombinant human ubiquitin-conjugating enzyme UbcH5c

英文题名：Structural analysis of recombinant human ubiquitin-conjugating enzyme UbcH5c

作者：Fangshu Wu[1];Junsheng Zhu[1];Honglin Li[1];Lili Zhu[1]

机构：[1]Shanghai Key Laboratory of New Drug Design,School of Pharmacy,East China University of Science and Technology,Shanghai 200237,China

年份：2017

卷号：7

期号：3

起止页码：390

中文期刊名：Acta Pharmaceutica Sinica B

外文期刊名：药学学报（英文版）

收录：CSTPCD;;Scopus;CSCD:【CSCD2017_2018】；PubMed;

基金：supported by the National Natural Science Foundation of China(grants 21372078,81302697 and 81230090);the National S&T Major Project of China(grant 2013ZX09507004);the Shanghai Committee of Science and Technology(grant 14431902400);the Fundamental Research Funds for the Central Universities

语种：英文

中文关键词：UbcH5c;NF-κB;Ubiquitination;Ubiquitin-conjugating enzyme;Crystal structure;Inflammatory target

外文关键词：UbcH5c;;NF-κB;;Ubiquitination;;Ubiquitin-conjugating enzyme;;Crystal structure;;Inflammatory target

摘要：UbcH5c belongs to the ubiquitin-conjugating enzyme family and plays an important role in catalyzing ubiquitination during TNF-α–triggered NF-κB activation. Therefore, UbcH5c is a potent therapeutic target for the treatment of inflammatory and autoimmune diseases induced by aberrant activation of NF-κB. In this study, we established a stable expression system for recombinant UbcH5c and solved the crystal structure of UbcH5c belonging to space group P22_12_1 with one molecule in the asymmetric unit. This study provides the basis for further study of UbcH5c including the design of UbcH5c inhibitors.
UbcH5c belongs to the ubiquitin-conjugating enzyme family and plays an important role in catalyzing ubiquitination during TNF-α–triggered NF-κB activation. Therefore, UbcH5c is a potent therapeutic target for the treatment of inflammatory and autoimmune diseases induced by aberrant activation of NF-κB. In this study, we established a stable expression system for recombinant UbcH5c and solved the crystal structure of UbcH5c belonging to space group P22_12_1 with one molecule in the asymmetric unit. This study provides the basis for further study of UbcH5c including the design of UbcH5c inhibitors.