文献类型：期刊文献

中文题名：离子排斥色谱法测定人体粪便中五种短链脂肪酸

英文题名：The detection of five SCFA in human's feces by HPIEC

作者：刘娟[1];杨柳[1];马浩[1];尹雪梅[1];施超欧[1]

机构：[1]华东理工大学分析测试中心,上海200237

年份：2013

卷号：32

期号：9

起止页码：104

中文期刊名：分析试验室

外文期刊名：Chinese Journal of Analysis Laboratory

收录：CSTPCD;;北大核心:【北大核心2011】；CSCD:【CSCD2013_2014】；

语种：中文

中文关键词：短链脂肪酸;人体粪便;离子排斥色谱

外文关键词：Short-chain fatty acids; Human feces; HPIEC

摘要：建立了一种离子排斥色谱分离测定人体粪便中5种短链脂肪酸（SCFA：乙酸、丙酸、异丁酸、丁酸、异戊酸）的方法。以体积分数0．1％磷酸为流动相、提取的样品在50℃下用ShodexRspakKC-811离子排斥柱分离，紫外检测器在210BITI处测定其中的有机酸。被测组分的浓度与其峰面积在2．5—500mg／L范围内呈良好的线性关系，相关系数R。≥0．999，各组分测定的相对标准偏差在2．3％-3．9％之间，平均加标回收率在85．5％-105．7％。
A method to determine the short-chain fatty acids in human feces was established, which is highly efficient, rapid, as well as accurate. The five kinds of short-chain fatty acids which existed in human feces （SCFA ： acetic acid, propionic acid, determined by ion-exclusion fraction is 0. 1% ） as the mo isobutyric acid, butyric acid, isovaleric acid ） were separated and chromatography. Under a temperature of 50 ℃, using phosphoric acid （volume bile phase, the extracted samnles have been separated by a Shodex Rspak KC -811ion exclusion column successfully. Meanwhile, those organic acids in them have been detected by a UV detector under a wavelength of 210 nm. Concentrations of the measured components and their peak areas both showed good linear relationships between 2.5 and 500 mg/L with correlation coefficient R2 of ≥ 0. 999, and the RSDs of mass concentrations of all the components were within the range of 2.3% - 3.9%. Average recoveries were from 85.5% to 105.7%. The method is simple, effective and accurate in the determination of several major SCFAs in human feces. Thus, it laid a strong foundation for the exploration of the physiological mechanisms which SCFA functioned in the normal guts.