文献类型：期刊文献

英文题名：A facile way to fabricate manganese phosphate self-assembled carbon networks as efficient electrochemical catalysts for real-time monitoring of superoxide anions released from HepG2 cells

作者：Cai, Xuan[1];Shi, Libo[1];Sun, Wenqian[1];Zhao, Hongli[1];Li, Hong[1];He, Haiyan[1];Lan, Minbo[1,2]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2018

卷号：102

起止页码：171

外文期刊名：BIOSENSORS & BIOELECTRONICS

收录：;EI(收录号：20174604392188);WOS:【SCI-EXPANDED(收录号:WOS:000424176600023)】；

基金：This research was financially supported by the Science and Technology Commission of Shanghai Municipality (STCSM, No. 16520710800) and the Fundamental Research Funds for the Central Universities (No. 222201717022).

语种：英文

外文关键词：Non-enzymatic sensor; Superoxide anion; Living cells; Manganese phosphate; Carbon materials

摘要：Quantification of superoxide anions (O-2(center dot-)) is significant in the monitoring of many serious diseases and the design of enzyme-mimic catalysts plays the main role in the development of non-enzymatic O-2(center dot-) sensors. Herein, we proposed a facile self-assembly process to synthesize manganese phosphate modified carbon networks using three kinds of widely-used carbon materials (MWCNTs, NGS and GO) as pillar connectors. Characterizations demonstrate that manganese phosphate is widely dispersed inside and on the surface of carbon networks without visible morphology. Meanwhile, all three kinds of synthesized catalysts were successfully immobilized on the screen-printed carbon electrodes to evaluate the electrochemical performance of fabricated sensors. The results indicate that sensors based on Mn-x(PO4)(y) modified MWCNTs exhibit high sensitivity with an extremely low detection limit of 0.127 mu M (S/N = 3) and a wide liner range of 0-1.817 mM (R-2 = 0.998). We further employed the recommended sensors in the real-time monitoring of HepG2 cells released O-2(center dot-) under the stimulating of Zymosan (20 mg/mL). Noticeably, the proposed sensors exhibit not only sensitive response but also stable current steps upon different addition of Zymosan. The calculated concentrations of cell-released O-2(center dot-) vary from 6.772 to 24.652 pM cell(-1) for the Zymosan amount used in this work. The established novel sensors display low background current and signal noises, thus holding unique advantages in the trace analysis of O-2(center dot-) in biological samples and in vivo environment.