文献类型：期刊文献

中文题名：面包酵母催化乙酰乙酸乙酯的不对称还原反应

英文题名：Asymmetry Bioreduction of Ethyl Acetoacetate Catalyzed by Baker's Yeast

作者：朱文洲[1];许建和[1];俞俊棠[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2000

卷号：26

期号：2

起止页码：154

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;国家哲学社会科学学术期刊数据库;EI(收录号：2000095315460);Scopus;北大核心:【北大核心1996】；CSCD:【CSCD2011_2012】；

基金：国家自然科学基金!2 97760 18

语种：中文

中文关键词：面包酵母;生物催化;乙酰乙酸乙酯;不对称还原

外文关键词：biocatalysis; asymmetric reduction ;baker's yeast; ethyl acetoacetate; ethyl ( S ) 3 hydryoxybutyrate

摘要：利用面包酵母催化的乙酰乙酸乙酯立体选择性还原反应 ,制备了光学活性的手性化合物—— ( S) - 3-羟基丁酸乙酯。分别考察了能源供体、蔗糖浓度、反应温度、底物加量和压榨酵母用量等因素对产物得率和对映体过量值的影响。结果表明 ,乙酰乙酸乙酯不对称生物还原反应的最适条件为 :能源供体 2 0 0 g/L蔗糖 ,反应温度 35℃ ,压榨酵母 1 70 g/L,底物加量 0 .2 mol/L。
Optically active ethyl ( S ) 3 hydroxybutyrate (E 3 HB), a useful chiral synthon, was prepared by stereoselective bioreduction of ethyl acetoacetate with pressed baker's yeast ( Saccharamyces cerevisiae). Effects of energy source, sucrose concentration, reaction temperature, substrate concentration and amount of pressed yeast on the yield and enantiometric excess ( ee ) of the product were examined respectively. The results showed that 200g/L of sucrose as energy source, 35℃, 170g/L of pressed yeast and 0.2mol/L of substrate were optimal for the asymmetric bioreduction of ethyl acetoacetate.