文献类型：期刊文献

英文题名：Engineering the methyltransferase through inactivation of the genK and genL leads to a significant increase of gentamicin C1a production in an industrial strain of Micromonospora echinospora 49-92S

作者：Xu, Feng[1];Zhang, Xinyu[1];Liu, Ling[1];Ke, Xiang[1];Wu, Jie[1];Guo, Yuanxin[1];Tian, Xiwei[1];Chu, Ju[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2022

卷号：45

期号：10

起止页码：1693

外文期刊名：BIOPROCESS AND BIOSYSTEMS ENGINEERING

收录：;EI(收录号：20223512667979);WOS:【SCI-EXPANDED(收录号:WOS:000847053900001)】；

基金：This work was financially supported by a grant from the National Key Research and Development Program of China (No. 2019YFA0904800). Meanwhile, this work was financially supported by an education cooperation fund from the Yihai Kerry Arawana Holdings Co., Ltd.

语种：英文

外文关键词：Micromonospora echinospora; Gentamicin C1a; Methyltransferase; GenK-genL; Metabolic flux analysis

摘要：In this study, a single-component high-yielding Micromonospora echinospora strain 49-92S-KL01 was constructed by deleting methyltransferase-encoding genes genK and genL. In 5-L fermentation trials, gentamicin C1a titers in the mutant strain were 3.22-fold higher than that in the parental strain (211 U/mL vs. 50 U/mL). The glycolysis pathway and tricarboxylic acid cycle fluxes were reduced by 26.8% and 26.6%, respectively, compared to the parental strain according to the metabolic flux analysis during the stationary phase, resulting in lower levels of energy supplements required for the cellular maintenance. Meanwhile, a significant enhancement in precursor (paromamine) accumulation and availability was observed in 49-92S-KL01 compared to parental strain. These results indicate that genK and genL significantly affect the synthesis of gentamicin C1a. In addition, this study provides a more rational strategy for gentamicin C1a production.