文献类型：期刊文献

中文题名：HS-SPME/GC-MS法测定北细辛挥发性成分

英文题名：Analysis of volatile components in Asarum heterotropoides by HS-SPME/GC-MS

作者：王风[1];李楠[1];李雪[1];钟灵艳[1];黄泽甜[1];胡坪[1]

机构：[1]华东理工大学化学与分子工程学院上海市功能性材料化学重点实验室,上海200237

年份：2021

卷号：51

期号：11

起止页码：1150

中文期刊名：日用化学工业

外文期刊名：China Surfactant Detergent & Cosmetics

收录：CSTPCD;;北大核心:【北大核心2020】；

基金：国家自然科学基金资助项目(81973285)。

语种：中文

中文关键词：北细辛;挥发性成分;顶空固相微萃取/气相色谱-质谱法

外文关键词：Asarum heterotropoides;volatile components;HS-SPME/GC-MS

摘要：建立了北细辛挥发性成分的顶空固相微萃取/气相色谱-质谱法(HS-SPME/GC-MS)分析方法,并对6个不同产地北细辛进行分析,利用谱库检索和保留指数对挥发性成分进行鉴定,从6个产地的北细辛中共鉴定出68种挥发性成分,挥发性成分中甲基丁香酚的相对含量最高,峰面积百分比为17.20%~33.80%。将HS-SPME/GC-MS法应用于北细辛挥发性成分的研究不仅能反映不同产地北细辛挥发性成分的整体差异,且方法简单快速,为北细辛挥发性的质量评价提供新思路。
Asarum is a kind of commonly used traditional Chinese medicine,and volatile oil is the main material basis for its efficacy.In traditional studies,the volatile oil is extracted by steam distillation and analyzed by GC-MS.Although the extraction method is easy to operate and has low cost,it is time-consuming,large sample consumption,high extraction temperature and easy thermal decomposition of active components.Moreover,xylene is often added in this method to promote the stratification of volatile oil,which brings great interference to the subsequent GC-MS analysis and component identification of volatile oil.Therefore,Solid Phase Microextration(SPME)technology that integrates extraction,purification,enrichment,and sampling functions was used to analyze the volatile oil of Asarum for the first time.The HS-SPME/GC-MS method was established to analyze Asarum heterotropoides,and the volatile components were identified by the NIST spectral library search and retention index.A total of 68 components are identified from the volatile oil of Asarum heterotropoides from 6 origins.The relative content of methyl eugenol is the highest among the volatile components with peak area percentages ranging from 17.20%to 33.80%.The application of HS-SPME/GC-MS method to the study of the volatile components in Asarum heterotropoides can reflect the overall differences,and the method is simple and rapid,providing new ideas for the quality evaluation of Asarum heterotropoides.