文献类型：期刊文献

英文题名：Affinity chromatography of urokinase using cefazoline as a ligand

作者：Cao, XJ; Wu, XY; Zhu, KY; Dai, GC

机构：[1]E China Univ Chem Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：1998

卷号：30

期号：6

起止页码：635

外文期刊名：ACTA BIOCHIMICA ET BIOPHYSICA SINICA

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000077163500020)】；

语种：英文

外文关键词：cefazoline; affinity chromatography; urokinase; agarose; epichlorohydrin

摘要：Sepharose 4B-based affinity adsorbent was prepared using cefazoline as a ligand, and epichlorohydrin as an activator. The density of the ligand in the affinity adsorbents was about 43 mu mol/g wet gel. The optimal adsorption pH for urokinase was 6.0, and the optimal adsorption salt concentration was 1.0 mol/L NaCl. The optimal conditions of elution was 0.1 mol/L glycine buffer, pH 9.0, containing 0.5 mol/L NaCl. A crude urokinase with specific activity 500 u/mg protein was purified on an affinity chromatography column (1 cm x 12 cm), yielding a product of specific activity of 49 300 u/mg with 78% recovery and 98.6-fold purification.