文献类型：期刊文献

中文题名：渗透压对于拟干酪乳杆菌发酵生产L-乳酸的影响

英文题名：Influence of osmotic stress on fermentative production of L-lactic acid by Lactobacillus paracasei

作者：张宁[1];田锡炜[1];王永红[1];储炬[1];张嗣良[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2014

卷号：35

期号：10

起止页码：98

中文期刊名：食品工业科技

外文期刊名：Science and Technology of Food Industry

收录：CSTPCD;;北大核心:【北大核心2011】；CSCD:【CSCD2013_2014】；

基金：国家重大科学仪器设备开发和应用专项(2012YQ15008709)

语种：中文

中文关键词：L-乳酸;拟干酪乳杆菌;渗透压;脯氨酸

外文关键词：L-lactic acid ; L. paracasei; osmotic pressure ; proline

摘要：研究了渗透压对于拟干酪乳杆菌（L．paracasei）发酵产L-乳酸的的影响。首先以NaCl作为渗透压调节剂研究渗透压对L．paracasei生长及产酸的影响，通过比较不同浓度NaCl（0-2mol／L），最终选择1mol／LNaCl作为渗透压保护剂的筛选条件。然后在该筛选条件下，研究了三种渗透压保护剂对L．paracasei产L-乳酸的作用，结果表明添加2g，L脯氨酸可提供最优的渗透压保护作用。最后通过5L罐批发酵验证上述结果，结果显示发酵24h后添加2g／L的脯氨酸能在接下来-段发酵时间内使得乳酸产率和葡萄糖消耗率分别比对照组提高50．34％和39．61％。此外，分析发酵结束时胞外主要副产物，结果表明添加脯氨酸后能够有效降低副产物的生成，提高L-乳酸的生产。本研究通过添加渗透压保护剂脯氨酸提高了乳酸的产量，从而为其他有机酸发酵提供了重要的借鉴意义。
The influence of osmotic pressure on the production of L-lactic acid by Lactobacillus paracasei was studied. First,NaCI was used as an osmotic regulator to study the influence of osmotic pressure on the growth and acid production of the Lactobacillus paracasei. By comparing different concentrations of NaCl（0-2mol/L）, lmol/L NaCI was chosed as an osmotic pressure filter condition. Then the role of three different osmoprotectants in the production of L-lactic acid was studied in this filter condition. Results indicated that 2g/L proline offered optimal osmoprotection. Finally,in tests of 5L batch fermentation,2g/L proline was added to the broth after 24h of fementation,and the productivity of L-lactic acid and consumption rate of glucose in phase II were observed to be increased by 50.34% and 39.61% ,respectively,as compared with the control. Moreover,the analysis of the main extracellular byproducts demonstrated that proline addition could decrease the formation of byproducts efficiently and increase the production of L-actic acid. This method of enhancing L-lactic acid production by the addition of an osmoprotectant might potentially provide an alternative approach for enhanced production of other organic acids.