文献类型：期刊文献

英文题名：Construction of a fluorescence turn-on probe for highly discriminating detection of cysteine

作者：Wang, Feiyi;An, Jiancai;Zhang, Lili;Zhao, Chunchang[1]

机构：[1]E China Univ Sci & Technol, Key Lab Adv Mat, Shanghai 200237, Peoples R China; E China Univ Sci & Technol, Inst Fine Chem, Shanghai 200237, Peoples R China

年份：2014

卷号：4

期号：96

起止页码：53437

外文期刊名：RSC ADVANCES

收录：;EI(收录号：20144500168373);WOS:【SCI-EXPANDED(收录号:WOS:000344468800009)】；

基金：We gratefully acknowledge the financial support by the National Science Foundation of China (grant no.: 21372083, 21172071, 21190033).

语种：英文

外文关键词：Fluorescence - Amino acids

摘要：It is still a challenge to construct probes for discriminating thiols due to the similar structure and reactivities of thiol-containing molecules. We have here developed a Cys specific probe by utilizing the remarkable difference in reactivity toward Cys, Hcy and GSH. The reaction between the designed probe and Cys produces an amino-substituted BODIPY, giving a yellow fluorescence turn-on response. The response to Hcy or GSH shows a red fluorescence turn-on signal, due to the formation of sulfenyl-substituted BODIPY. These distinct fluorescence turn-on responses allow Cys to be distinguished from Hcy and GSH. This probe was also utilized for detection of Cys in living cells and monitoring cystathionine gamma-lyase activity in vitro.