文献类型：期刊文献

中文题名：Breaking or following the membrane-targeting mechanism:Exploring the antibacterial mechanism of host defense peptide mimicking poly(2-oxazoline)s

作者：Chengzhi Dai[1];Min Zhou[1];Weinan Jiang[1];Ximian Xiao[1];Jingcheng Zou[1];Yuxin Qian[1];Zihao Cong[1];Zhemin Ji[1];Longqiang Liu[1];Jiayang Xie[1];Zhongqian Qiao[1];Runhui Liu[1,2]

机构：[1]State Key Laboratory of Bioreactor Engineering,Frontiers Science Center for Materiobiology and Dynamic Chemistry,Key Laboratory of Specially Functional Polymeric Materials and Related Technology(ECUST)Ministry of Education,School of Materials Science and Engineering,East China University of Science and Technology,Shanghai,200237,China;[2]State Key Laboratory of Molecular Engineering of Polymers(Fudan University),Shanghai,200438,China

年份：2020

卷号：59

期号：24

起止页码：220

中文期刊名：Journal of Materials Science & Technology

外文期刊名：材料科学技术（英文版）

收录：CSTPCD;;Scopus;CSCD:【CSCD2019_2020】；PubMed;

基金：financially supported by the Natural Science Foundation of Shanghai(18ZR1410300);the National Natural Science Foundation of China(No.21861162010,21774031);the National Key Research and Development Program of China(No.2016YFC1100401);the Research Program of State Key Laboratory of Bioreactor Engineering;the Fundamental Research Funds for the Central Universities(No.22221818014,50321041917001)。

语种：英文

中文关键词：Poly(2-oxazoline);Host defense peptide;Antibacterial mechanism;Membrane-targeting;Antimicrobial resistance

摘要：Peptides exert important biological functions but their application is hindered by their susceptibility to proteolysis and poor stability in vivo.Thus,functional peptide mimics have drawn a great deal of attention to address this challenge.Poly(2-oxazoline)s,a class of biocompatible and proteolysis-resistant polymer,can work as host defense peptide mimics without following the general membrane-targeting mechanism as shown in our previous work.This observation encouraged us to figure out if poly(2-oxazoline)s are special and break the general membrane-targeting mechanism of host defense peptides and their mimics.In this study,we aimed at the connection between structure and antibacterial mechanism of poly(2-oxazoline)s.A new γ-aminobutyric acid(GABA)-pendent poly(2-oxazoline)was synthesized and investigated to compare with glycine-pendent poly(2-oxazoline)in our previous study,with the former polymer has two extra CH2 groups in the sidechain to increase the hydrophobicity and amphiphilicity.Membrane depolarization assay suggested that incorporating two more CH2 groups into the sidechain of poly(2-oxazoline)resulted in a mechanism switch from DNA-targeting to membrane-targeting,which was supported by the slow time-kill kinetics and slightly distorted and sunken membrane morphology.Besides,GABA-pendent poly(2-oxazoline)showed potent activity against methicillin-resistant S.aureus and low hemolysis on human red blood cells.Moreover,repeated use of the antimicrobial poly(2-oxazoline)did not stimulate bacteria to obtain resistance,which was an obvious advantage of membrane-targeting antimicrobial agents.