文献类型：期刊文献

英文题名：Aminopropyl-functionalized silicas synthesized by W/O microemulsion for immobilization of penicillin G acylase

作者：Shi, Bianfang[1];Wang, Yunsong[1];Guo, Yanglong[1];Wang, Yanqin[1];Wang, Ying[1];Guo, Yun[1];Zhang, Zhigang[1];Liu, Xiaohui[1];Lu, Guanzhong[1]

机构：[1]E China Univ Sci & Technol, Res Inst Ind Catalysis, Adv Mat Lab, Shanghai 200237, Peoples R China

年份：2009

卷号：148

期号：1-2

起止页码：184

外文期刊名：CATALYSIS TODAY

收录：;EI(收录号：20100312648117);WOS:【SCI-EXPANDED(收录号:WOS:000271830100031)】；

基金：This project was supported financially by the National Basic Research Program of China (2004CB719500), Science and Technology Commission of Shanghai Municipality (06DJ14006) and Special Foundation for Ph.D. Education by Ministry of Education of China (20070251016).

语种：英文

外文关键词：Aminopropyl-functionalized silicas; Microemulsion; Immobilization; Penicillin G acylase

摘要：The a minopropyl-functionalized silicas (APFS) with higher loading of amino groups was synthesized by the co-condensation of tetraethylorthosilicate (TEOS) and gamma-aminopropyltriethoxysilane (APTES) in W/O microemulsion. Thus synthesized APFS was characterized by FT-IR spectroscopy, thermogravimetry, element analysis, solid state C-13 and Si-29 MAS NMR, TEM, and N-2 sorption. The results show that the aminopropyl groups were condensed as the part of the silicate framework, and the amino contents could be adjusted by changing the volume ratio of APTES to TEOS in W/O microemulsion. APFS has been firstly used as the support for the immobilization of penicillin G acylase after activation with glutaraldehyde. Effects of the volume ratio of APTES to TEOS on the physico-chemical properties of APFS and the performance of immobilized penicillin G acylase were systematically investigated. Penicillin G acylase immobilized on APFS with the volume ratio of APTES/TEOS = 1/9 (1.77 mmol g(-1) amino groups) behaves higher specific activity of 2759 IU g(-1) and higher operational stability of 86% of the initial specific activity after recycled for 5 times, and the immobilization yield of 97%. (C) 2009 Elsevier B.V. All rights reserved.