文献类型：专利

英文题名：Ferritin heavy chain subunits nanoparticles useful in hybrid protein nanoparticles for preparation of drug nanoparticles for targeting tumor cells, comprises ferritin heavy polypeptide 1 N-terminal modified with penetratin peptide

作者：HUANG P;CAO X;WANG J

机构：[1]UNIV EAST CHINA SCI & TECHNOLOGY

申请号：CN104017089-A

申请日：2014-06-25

公开日：2014-09-03

语种：英文

收录：DERWENT

摘要：NOVELTY - A ferritin heavy chain subunits nanoparticle comprises ferritin heavy polypeptide 1 (FTH1) N-terminal modified with penetratin peptide. USE - As ferritin heavy chain subunits nanoparticle useful in hybrid protein nanoparticles used in preparation of nanoparticle drug for targeting tumor cells (claimed). ADVANTAGE - The N terminal heavy chain subunit ferritin nanoparticles is modified with short peptides having different charge properties and simultaneously refolding nanoparticles modified with epidermal growth factor (EGF), and self-assembling in vitro. The formed hybrid protein nanoparticles have a strong cellular penetration and tumor target cell strong binding. The subsequent selection of drug therapy for targeting tumor cells provides a very good model, and has good prospects. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for the following: (1) a hybrid protein nanoparticles, comprising N-terminal modified penetratin peptide subunit ferritin heavy chain and the N-terminal modified nanoparticles with epidermal growth factor (EGF) ferritin heavy chain subunits of nanoparticles; and (2) preparation of the hybrid protein nanoparticles, involving: (1a) construction of gene engineering strain: constructing expression of N-terminal modifications of penetrating peptide subunits of ferritin heavy chain fusion protein and N-terminal modifications of EGF ferritin heavy chain fusion protein subunit gene engineering strain, where a expression vector is pET-28 (+) of a host strain Escherichia coli; (2a) harvesting the fusion protein: inoculating the step (1a) gene engineering strain to LB culture medium, adding in culture IPTG induction expression, crushing cells, centrifuging, to obtain harvested expression of N-terminal modifications of trans-membrane ferritin heavy chain subunit peptide fusion protein and N-terminal modifications of EGF ferritin heavy chain subunit fusion protein; and (3a) self-assembling hybrid protein nanoparticles: placing the in vitro expression obtained in the step (2a), into dialysis liquid, gradient dialysis renaturation penetrating, and harvesting to obtain self-assembled hybrid protein nanoparticles.