文献类型：期刊文献

中文题名：正交试验优化胶原多肽螯合钙的制备工艺

英文题名：Optimization of Preparation of Collagen Polypeptide Chelated Calcium by Orthogonal Array Design

作者：彭巧云[1];沈菊泉[2];魏东芝[1];马志英[2];沈亚领[1]

机构：[1]华东理工大学生物工程学院,生物反应器工程国家重点实验室,上海200237;[2]上海市食品研究所,上海200235

年份：2013

卷号：34

期号：8

起止页码：94

中文期刊名：食品科学

外文期刊名：Food Science

收录：CSTPCD;;Scopus;北大核心:【北大核心2011】；CSCD:【CSCD2013_2014】；

基金：上海市科学技术委员会应用技术开发专项(08-120)

语种：中文

中文关键词：胶原多肽;螯合率;骨钙;骨密度

外文关键词：collagen polypeptide; chelating rate; bone calcium; bone mineral density

摘要：以胶原多肽及氯化钙为原料制备胶原多肽螯合钙并确定其最佳制备工艺,结果表明:胶原多肽螯合钙最适的螯合条件为钙元素和胶原多肽的质量比为13:100、pH6.0、时间30min、温度30℃。在该最佳螯合条件下得到的胶原多肽螯合钙的螯合率达到77.7mg/g。傅里叶变换红外光谱分析结果表明胶原多肽螯合钙是一种新的物质,后期的动物实验也证明了胶原多肽螯合钙具有增加骨密度的功能。
This work reports the application of orthogonal array design to explore the optimum chelating conditions of food-grade calciumchloride with collagen polypeptide. The optimal chelating conditions were found to be reaction at 30 ℃ and an initial pH of 6.0 for 30 min with a calcium-to-collagen polypeptide mass ratio of 13：100. Under the optimized conditions, the calcium-chelating efficiency of collagen polypeptides was 77.7 mg/g. FTIR analysis demonstrated that we successfully obtained collagen polypeptide chelated calcium as a new material. Moreover, the chelated calcium was found to increase bone density in animal studies.