文献类型：期刊文献

英文题名：Improvement of 1,3-propanediol production in Klebsiella pneumoniae by moderate expression of puuC (encoding an aldehyde dehydrogenase)

作者：Zhu, Chengqian[1];Jiang, Xiao[1];Zhang, Yongqiang[1];Lin, Jie[1];Fu, Shuilin[1];Gong, Heng[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2015

卷号：37

期号：9

起止页码：1783

外文期刊名：BIOTECHNOLOGY LETTERS

收录：;EI(收录号：20152000842378);WOS:【SCI-EXPANDED(收录号:WOS:000360432100008)】；

基金：This work was supported by the National Natural Science Foundation of China under Grant No. 31271862.

语种：英文

外文关键词：Aldehyde dehydrogenase; 3-Hydroxypropionic acid; Klebsiella pneumoniae; 1,3-Propanediol; puuC

摘要：To improve 1,3-propanediol production in Klebsiella pneumoniae, the effects of puuC expression in lactate- and lactate/2,3-butanediol-deficient strains were assessed. Overexpression of puuC (encoding an aldehyde dehydrogenase) inhibited 1,3-propanediol production and increased 3-hydroxypropionic acid formation in both lactate- and lactate/2,3-butanediol-deficient strains. An improvement in 1,3-propanediol production was only achieved in a lactate-deficient strain via moderate expression of puuC; at the end of the fermentation, 1,3-propanediol productivity increased by 14 % compared with the control. Further comparative analysis of the metabolic flux distributions in different strains indicated that 3-hydroxypropionic acid formation could play a considerable role in cell metabolism in K. pneumoniae. An improvement in 3-hydroxypropionic acid formation would be beneficial for cell metabolism, which can be accomplished by enhancing 1,3-propanediol productivity in a lactate-deficient strain via moderate expression of puuC.