文献类型：期刊文献

英文题名：Reduced methanol input induces increased protein output by AOX1 promoter in a trans-acting elements engineered Pichia pastoris

作者：Wang, Jinjia[1];Wang, Xiaolong[1];Shi, Lei[1];Zhang, Yuanxing[1,2,3];Zhou, Xiangshan[1,2];Cai, Menghao[1,2]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Biotechnol, 130 Meilong Rd, Shanghai 200237, Peoples R China;[3]Shanghai Collaborat Innovat Ctr Biomfg, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2018

卷号：45

期号：1

起止页码：25

外文期刊名：JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

收录：;EI(收录号：20242616369621);WOS:【SCI-EXPANDED(收录号:WOS:000419898200003)】；

基金：This work was supported by the Shanghai Science and Technology Innovation Action Plan (17JC1402400), Fundamental Research Funds for the Central Universities (22A201514040), National Special Fund for State Key Laboratory of Bioreactor Engineering (2060204) and Talent Program of School of Biotechnology in East China University of Science and Technology.

语种：英文

外文关键词：Pichia pastoris; Insulin precursor; Methanol induction; AOX1 promoter; Protein expression

摘要：High oxygen consumption and heat release caused by methanol catabolism usually bring difficulties to industrial scale-up and cost for protein expression driven by methanol-induced AOX1 promoter in Pichia pastoris. Here, reduced methanol feeding levels were investigated for expression of insulin precursor in a trans-acting elements engineered P. pastoris strain MF1-IP. Insulin precursor expression level reached 6.69 g/(L supernatant) at the methanol feeding rate of 6.67 mL/(h.L broth), which was 59% higher than that in the wild-type strain WT-IP at the methanol feeding rate of 12 mL/(h.L broth). Correspondingly, the insulin precursor expression level in fermentation broth and maximum specific insulin precursor production rate was 137 and 77% higher than the WT-IP, respectively. However, oxygen consumption and heat evolution were reduced, and the highest oxygen consumption rate and heat evolution rate of the MF1-IP were 18.0 and 37.7% lower than the WT-IP, respectively.