文献类型：期刊文献

中文题名：酶法合成2＇－脱氧－5－氟尿苷

英文题名：Enzymatic Synthesis of2'-Deoxy-5-Fluoro-Uridine

作者：周长林[1];邱蔚然[1];俞俊棠[1]

机构：[1]华东理工大学生化工程研究所

年份：1995

卷号：2

期号：4

起止页码：24

中文期刊名：药物生物技术

外文期刊名：Pharmaceutical Biotechnology

收录：CSTPCD;;CSCD:【CSCD_E2011_2012】；

语种：中文

中文关键词：2'-脱氧-5-氟尿苷;氟尿嘧啶;酶法合成;核苷磷酸化酶

外文关键词：Deoxy-5-fluoro-uridine; Fluorouracil; Enzymatic synthesis; Nucleooide phosphorylase

摘要：本文采用大肠杆菌核苷磷酸化酶，以５－氟尿嘧啶（ＦＵ）、２＇－脱氧尿苷（ｄＵ）和磷酸盐为底物酶法合成２＇－脱氧－５－氟尿苷，实验结果表明，含１０ｍｍｏｌ／ＬｄＵ、３０ｍｍｏｌ／ＬＦＵ、１０－３０ｍｍｏｌ／Ｌ磷酸盐的混合物加入５％培养２４ｈ的大肠杆菌湿菌体于ｐＨ７．０反应３ｈ，底物ｄＵ的转化率可达４５．９％。
he enzymatic synthesis of2'-deoxy-5-fluoro-urluine with E. coli nucleoside phosphorylase was stud-ied. The substrates of it were 5-fluorouracil,2'-deoxy-uridine and potassium phosphate. The experi-ment showed that the optimum substrates concentration was 10 mmol/L dU , 30 mmol/L FU,10-30mmol/L potassium phosphate. When we added 5%(w/V) E. coli wet cell cultured for 24h to the re-acting mixture and adjusted pH to 7.0 with KOH, the biosynthesis took place at 70℃ for 3 h effective-ly and 45.9%dU was converted to FdU.