文献类型：期刊文献

中文题名：混合溶剂系统中固定化脂肪酶对酮基布洛芬的催化酯化反应

英文题名：Enantioselective Esterification of Ketoprofen by Immobilized Lipase in Mixed Solvent Systems

作者：刘军民[1];许建和[1];刘幽燕[1];许学书[1];袁勤生[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室

年份：1998

卷号：19

期号：12

起止页码：1959

中文期刊名：高等学校化学学报

外文期刊名：Chemical Journal of Chinese Universities

收录：CSTPCD;;Scopus;北大核心:【北大核心1996】；CSCD:【CSCD2011_2012】；

基金：国家自然科学基金;国家教育委员会留学回国人员基金

语种：中文

中文关键词：酮基布洛芬;固定化脂肪酶;对映选择性;酯化;拆分

外文关键词：Ketoprofen, Immobilized lipase, Enantioselective esterification, Mixed solvent systems

摘要：以硅藻土吸附的脂肪酶为催化剂，对外消旋酮基布洛芬［２－（３－苯甲酰苯基）丙酸］进行对映选择性酯化反应；考察了不同的脂肪酶制剂，固定化时所加缓冲液的体积与ｐＨ值，酰基受体（醇）的种类以及混合溶剂系统的组成等因素对酶活性的影响．结果表明，在所考察的７种脂肪酶中，以ＬｉｐａｓｅＯＦ的酯化活性最高；用硅藻土吸附固定化酶时，缓冲溶液的最适ｐＨ为７．０左右，每克酶粉加１．０ｍＬ缓冲溶液为最佳；固定化酶催化酯化的活性比游离的脂肪酶高．在酮基布洛芬与不同酰基受体（醇）的酶促酯化反应中，以丙醇的反应速度为最快．在由一种主溶剂与一种助溶剂组成的混合溶剂系统中，酶促酯化的速度要比在单一的主溶剂或助溶剂系统中快．当以ｌｇＰ值较大的环己烷或异辛烷等为主溶剂，甲苯为助溶剂时，脂肪酶催化酮基布洛芬酯化反应的活性最高．
Enantioselective esterification of racemic ketoprofen[2 (3 benzoylphenyl) propionic acid] was carried out in organic solvents by using celite adsorbed lipase as the catalyst. Factors influencing the activity of the immobilized lipase had been investigated. Among the seven lipases examined, the esterification activity of Lipase OF(from Candida rugosa) was the highest; the activity of immobilized lipase was improved as compared with free lipase; the optimum volume of buffer(0.1 mol/L Na 2HPO 4 NaH 2PO 4, pH 7.0) for enzyme immobilization was 1 mL pergram lipase. Among the acyl receptors such as methanol, ethanol, n propanol, n butanol, n octanol, iso propanol and 2 ethylene chlorohydrin, n propanol was the best; In the mixed solvent system composed of a main solvent and a cosolvent, the esterification activity of lipase was higher than that in the single solvent system composed of the corresponding main solvent or cosolvent. The esterification activity of lipase was the highest when using cyclohexane or iso octane, both of which have high lg P values, as main solvents and toluene as cosolvent.