文献类型：期刊文献

英文题名：Optimization of a broth conductivity controlling strategy directed by an online viable biomass sensor for enhancing Taxus cell growth rate and Taxol productivity

作者：Wang, Ze-Jian[1,2];Zhang, Wei[1];Zhang, Jian-Wen[1];Guo, Mei-Jin[1];Zhuang, Ying-ping[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, POB 329,130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Delft Univ Technol, Dept Biotechnol, Julianalaan 67, NL-2628 BC Delft, Netherlands

年份：2016

卷号：6

期号：47

起止页码：40631

外文期刊名：RSC ADVANCES

收录：;EI(收录号：20162102408958);WOS:【SCI-EXPANDED(收录号:WOS:000375270600002)】；

基金：This work was financially supported by a grant from the Major State Basic Research Development Program of China (863 Program), No. 2012AA021201, and 973 Program No. 2013CB733600. We would also like to warmly thank Guangdong Kelun Pharmaceutical Company for donating the industrial strain of Taxus chinensis var. mairei.

语种：英文

外文关键词：Plant cell culture - Growth kinetics - Capacitance - Cells - Growth rate

摘要：Online process control is always an important and challenging technique for anti-cancer Taxol (paclitaxel) production by a plant cell suspension culture due to its slow growth rate, variability and intense sensitivity to environmental factors. In this work, we investigated the effects of a constant broth conductivity controlling strategy directed by an online viable biomass sensor on Taxol productivity of Taxus chinensis var. mairei in suspension cultivation. The viable cell capacitance was effectively used for detecting Taxus cell concentration online and for optimal feeding of the elicitor to stimulate Taxol production. An increase in conductivity has a negative effect on cell growth and elongation. With the optimal broth conductivity constantly controlled at 2.6 mS cm(-1) and methyl jasmonate at 6 mmol g(-1) cell, the Taxus cell growth rate and Taxol production reached their highest value at 0.87 g d(-1) and 76.2 mg L-1 at 8 days after methyl jasmonate addition, and productivity reached its highest at 850 mg per g DCW per d, which was much higher than that under the higher conductivity conditions. Therefore, a substrate real-time feedback control strategy based on capacitance and conductivity must be an effective method for enhancing Taxol productivity and can be used for the scale up of a plant cell suspension culture.