文献类型：期刊文献

英文题名：Direct electrochemistry of hemoglobin immobilized on siliceous mesostructured cellular foam

作者：Li, Jinxia[1,2];Zhou, Lihui[1,2];Han, Xia[1,2];Hu, Jun[1,2];Liu, Honglai[1,2];Xu, Jian[3]

机构：[1]E China Univ Sci & Technol, Key Lab Adv Mat, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Dept Chem, Shanghai 200237, Peoples R China;[3]Shanghai Inst Measurement & Testing Technol, Shanghai 201203, Peoples R China

年份：2009

卷号：138

期号：2

起止页码：545

外文期刊名：SENSORS AND ACTUATORS B-CHEMICAL

收录：;EI(收录号：20091712047901);WOS:【SCI-EXPANDED(收录号:WOS:000265893800024)】；

基金：This work is supported by the National Natural Science Foundation of China (Project Nos. 20736002 and 20776045), Program for Changjiang Scholars and Innovative Research Team in University (No. IRT0721).

语种：英文

外文关键词：Hemoglobin; Direct electrochemistry; Mesostructured cellular foams; Immobilization of protein

摘要：The direct electrochemistry of hemoglobin (HI)) immobilized on the siliceous mesostructured cellular foam (MCF) modified glassy carbon electrode (GC) was presented. Using a simple physical adsorption method. Hb was immobilized on MCF and the influences of concentration of Hb and the pH value of solution were investigated. UV-vis and FTIR spectra displayed that Hb maintained its natural structure inside the pores of MCF. The electrochemical property of Hb/MCF/GC modified electrode was studied by cyclic voltammetry. A couple of quasi-reversible redox peaks of Hb were observed on the Hb/MCF/GC electrode at about -0.396V and -0.321V in PBS (pH 7.0). The results indicated that MCF provided a favorable microenvironment for immobilization of Hb and facilitated the electron transfer between Hb and electrode surface. (C) 2009 Elsevier B.V. All rights reserved