文献类型：期刊文献

英文题名：Altered let-7 expression in Myasthenia gravis and let-7c mediated regulation of IL-10 by directly targeting IL-10 in Jurkat cells

作者：Jiang, Lin[2,3];Cheng, Zhuoan[2,3];Qiu, Shaobo[1];Que, Zujun[2,3];Bao, Wenjing[4];Jiang, Chao[1];Zou, Fangyuan[2,3];Liu, Ping[1];Liu, Jianwen[2,3]

机构：[1]Shanghai Univ Tradit Chinese Med, Longhua Hosp, Shanghai 200032, Peoples R China;[2]E China Univ Sci & Technol, Sch Pharm, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[3]E China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab New Drug Design, Shanghai 200237, Peoples R China;[4]Liaoning Univ Tradit Chinese Med, China Affiliated Hosp, Liaoning 110032, Peoples R China

年份：2012

卷号：14

期号：2

起止页码：217

外文期刊名：INTERNATIONAL IMMUNOPHARMACOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000308682100014)】；

基金：This work was supported by Shanghai Science and Technology Commission (No. 10JC1414500) and Shanghai Committee of Science and Technology (grant 11DZ2260600).

语种：英文

外文关键词：Myasthenia gravis; let-7; let-7c; IL-10

摘要：Myasthenia gravis (MG) is a T cell-dependent and B cell-mediated autoimmune disease of neuromuscular junctions and cytokines may play a crucial role in the pathogenesis and perpetuation of MG. MicroRNAs (miRNAs) have been implicated as fine-tuning regulators controlling diverse biological processes at the level of posttranscriptional repression. Dysregulation of miRNAs has been described in various disease states. In this study, miRNA microarrays identified let-7 family to be decreased in peripheral blood mononuclear cells (PBMCs) from MG patients compared to the healthy controls. We next demonstrated the differential expression of let-7 family in larger samples by quantitative real-time PCR. Using a combination of bioinformatics and molecular approaches, we confirmed IL-10 as a target for let-7c. IL-10 expression also showed a negative correlation with let-7c expression in PBMCs from MG patients. Further experiments revealed that induced levels of IL-10 were inversely related to let-7c levels. We also showed that let-7c could regulate IL-10 expression in Jurkat cells. In summary, our results suggest that abnormal expression/regulation of microRNAs may contribute to or be indicative of the initiation and progression of MG. (C) 2012 Elsevier B.V. All rights reserved.