文献类型：期刊文献

英文题名：Rational Engineering of a Dynamic, Entropy-Driven DNA Nanomachine for Intracellular MicroRNA Imaging

作者：Liang, Cheng-Pin[1];Ma, Pei-Qiang[1];Liu, Hui[1,2];Guo, Xinggang[1,2];Yin, Bin-Cheng[1];Ye, Bang-Ce[1,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Lab Biosyst & Microanal, Shanghai 200237, Peoples R China;[2]Eastern Hepatobiliary Surg Hosp, Dept Hepat Surg 3, Shanghai 200438, Peoples R China;[3]Shihezi Univ, Sch Chem & Chem Engn, Xinjiang 832000, Peoples R China

年份：2017

卷号：56

期号：31

起止页码：9077

外文期刊名：ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

收录：;EI(收录号：20172703877407);WOS:【SCI-EXPANDED(收录号:WOS:000424260200024)】；

基金：This work was jointly supported by the National Natural Science Foundation of China (grant 21335003), the Science Fund for Creative Research Groups (grant 21421004), the National Natural Science Foundation of China (grants 21675052, 21575089, 81271649), the Fundamental Research Funds for the Central Universities, and Programme of Introducing Talents of Discipline to Universities (grant B16017).

语种：英文

外文关键词：catalytic reactions; DNA nanomachines; gold nanoparticles; microRNA; sensors

摘要：We rationally engineered an elegant entropy-driven DNA nanomachine with three-dimensional track and applied it for intracellular miRNAs imaging. The proposed nanomachine is activated by target miRNA binding to drive a walking leg tethered to gold nanoparticle with a high density of DNA substrates. The autonomous and progressive walk on the DNA track via the entropy-driven catalytic reaction of intramolecular toehold-mediated strand migration leads to continuous disassembly of DNA substrates, accompanied by the recovery of fluorescence signal due to the specific release of a dye-labeled substrate from DNA track. Our nanomachine outperforms the conventional intermolecular reaction-based gold nanoparticle design in the context of an improved sensitivity and kinetics, attributed to the enhanced local effective concentrations of working DNA components from the proximity-induced intramolecular reaction. Moreover, the nanomachine was applied for miRNA imaging inside living cells.