文献类型：期刊文献

英文题名：Sandwich-type electrochemical aptasensor based on polydopamine-Au-metal ions as signal label and coralloid Au-conductive carbon architecture-modified electrode for the simultaneous detection of cardiac troponin I and myoglobin

作者：Chen, Kaicha[1];Zhao, Hongli[1];Wang, Zhenxing[1];Zhou, Fangfang;Lan, Minbo[1,2]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Res Ctr Anal & Test, Shanghai 200237, Peoples R China

年份：2023

卷号：390

外文期刊名：SENSORS AND ACTUATORS B-CHEMICAL

收录：;EI(收录号：20232614297926);WOS:【SCI-EXPANDED(收录号:WOS:001054606900001)】；

基金：This work was supported by the Science and Technology Commission of Shanghai Municipality (STCSM, 20520712500; 20392002300) , and the Fundamental Research Funds for the Central Universities (JKJ01231718) .

语种：英文

外文关键词：Cardiac troponin I; Myoglobin; Polydopamine; Metal ions; Electrochemical aptasensor

摘要：The determination of cardiac troponin I (cTnI) and myoglobin (Myo) is increasingly significant considering their potential in acute myocardial infarction (AMI) diagnosis. Here, we constructed a sandwich-type electrochemical aptasensor for the simultaneous detection of cTnI and Myo. The polydopamine-Au-metal ions (PDA-Au-Pb2+/ Cu2+) were synthesized and used as the labels of second aptamers (TrO6 and S232). The labels displayed different peaks at different potential sites, thus making the simultaneous detection method available. Meanwhile, the synthesized zirconium-based carbon architecture (CC) was well-conductive originating from the annealing process. After the modification of CC on the bare electrode (screen-printed carbon electrode, SPCE), the coralloid Au (CAu) was further decorated on the electrode by electro-deposition, thus forming CAu/CC/SPCE with high conductivity. Hence, the aptasensor proposed here showed admirable simultaneous quantitative analysis performance towards cTnI and Myo with wide linear ranges (1 & mu;g/mL-0.01 pg/mL, 1 & mu;g/mL-0.1 pg/mL) and low detection limits (3.8 fg/mL, 35 fg/mL). These results demonstrated that the aptasensor held the potential application in clinical diagnosis.