文献类型：期刊文献

英文题名：Novel Real-Time PCR Method Based on Growth Hormone Gene for Identification of Salmonidae Ingredient in Food

作者：Li, Xiang[1];Li, Jinbo[1,2];Zhang, Shuya[1];He, Yuping[1];Pan, Liangwen[1]

机构：[1]Shanghai Entry Exit Inspect & Quarantine Bur, GMO Detect Lab, Shanghai 200135, Peoples R China;[2]E China Univ Sci & Technol, Inst Biotechnol, Shanghai 200237, Peoples R China

年份：2013

卷号：61

期号：21

起止页码：5170

外文期刊名：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

收录：;EI(收录号：20132316399474);WOS:【SCI-EXPANDED(收录号:WOS:000319856900025)】；

基金：This work was supported by grants from the Shanghai Special Fund for Technical Barriers to Trade (12TBT011).

语种：英文

外文关键词：Salmonidae; real-time PCR; growth hormone gene; species identification; food

摘要：To avoid fraudulent substitutions in fish markets, the proper methods are needed to test the authenticity of the ingredients. As a preferable methodology, a quantitative real-time polymerase chain reaction (qPCR) method was used in this study to identify species from the Salmonidae family based on the salmon growth hormone gene. Fish samples of six genera from the Salmonidae family were tested to identify the specificity, sensitivity, and applicability of the established method. Results showed that the method was highly specific for salmonid detection. Ct values were obtained only from 31 Salmonidae fish species samples. The relative and absolute limits of detection were 0.01% and 25 pg of genomic DNA, respectively, which could meet with the requirements of routine detections. To test the applicability of the method, the content of salmonid ingredients in 16 commercial food products was quantified from standard curves constructed from DNA of two Salmonidae species. The results revealed that the salmonid ingredient was detected in 12 samples, indicating that 25% of the labels are inauthentic. These results demonstrate that the developed qPCR method is suitable for identification of Salmonidae ingredients.