文献类型：期刊文献

中文题名：顺磁性脂质体包封率测定方法的研究

英文题名：Study on entrapment efficiency of paramagnetic liposomes

作者：戎丽娜[1];张敏[1];徐云龙[1];钱秀珍[1];吴秋芳[2];杨景辉[2]

机构：[1]华东理工大学材料科学与工程学院,超细材料制备与应用教育部重点实验室,上海200237;[2]国家超细粉末工程研究中心,上海200237

年份：2010

卷号：30

期号：8

起止页码：1435

中文期刊名：药物分析杂志

外文期刊名：Chinese Journal of Pharmaceutical Analysis

收录：CSTPCD;;北大核心:【北大核心2008】；CSCD:【CSCD2011_2012】；

基金：上海市纳米科技专项(0752nm018)资助;国家863计划项目(2007A-A02Z4B4)

语种：中文

中文关键词：Gd-DTPA;脂质体;包封率;透析法;超速离心法;超滤法

外文关键词：Gd-DTPA; liposome; entrapment efficiency; dialysis; ultracentrifugation; ultrafiltration

摘要：目的:建立Gd-DTPA顺磁性脂质体包封率的测定方法。方法:采用薄膜分散-高压均质法制备顺磁性(Gd-DTPA)脂质体。分别采用透析法、超速离心法、超滤离心法分离脂质体和游离药物,并用电感耦合等离子体-原子发射光谱法(ICP-AES)测定其中钆的含量,计算脂质体包封率。结果:ICP-AES法测定钆浓度在2～10mg·L-1范围内线性关系良好(r=0.9996);高、中、低3种浓度的日内和日间精密度试验的RSD均小于1%;消解方法的平均回收率(98.1%～100.3%)符合要求;3种包封率测定方法中超滤离心法测得的包封率最高,透析法测得的包封率最低。结论:超滤离心法结合ICP-AES法快捷、准确,适用于Gd-DTPA顺磁性脂质体包封率的测定。
Objective：To establish a method for determination of the entrapment efficiency of paramagnetic liposomes.Methods：The paramagnetic（Gd-DTPA） liposomes were prepared by thin film dispersion-high pressure homogenization.The liposomes and free drug were separated by dialysis,ultracentrifugation and ultrafiltration,then Gd concentration were determined by inductive coupled plasma-atomic emission spectrometry（ICP-AES）,and then the entrapment efficiency of the liposomes was calculated.Results：The calibration curve was linear（r=0.9996） with in the range of 2-10 mg·L-1 for gadolinium determined by ICP-AES.The RSDs of the intra-day and inter-day were less than 1%,and the recoveries of the digesting method were 98.1%-100.3%.Among the three methods,the entrapment efficiency determined by ultrafiltration was the highest,and the entrapment efficiency determined by dialysis was the lowest.Conclusion：The method of ultrafiltration with ICP-AES is rapid,accurate and reproducible.It is suitable for determining the entrapment efficiency of the paramagnetic liposomes.