文献类型：期刊文献

英文题名：Combining adaptive laboratory evolution and metabolic engineering for efficient erythritol production from crude glycerol by Yarrowia lipolytica

作者：Zhao, Ling-Xuan[1]; Li, Jiawei[2]; Wang, Ya-Ting[1]; Zhu, Qin-Yi[1]; Chen, Jun[1]; Liu, Zhi-Jie[3]; Liu, Feng[1]; Hua, Qiang[1]

机构：[1] State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China; [2] State Key Laboratory of Microbial Metabolism, and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China; [3] Key Laboratory of Fermentation Engineering [Ministry of Education], Cooperative Innovation Center of Industrial Fermentation [Ministry of Education & Hubei Province], Hubei Key Laboratory of Industrial Microbiology, Hubei University of Technology, Wuhan, 430068, China

年份：2026

卷号：419

起止页码：114

外文期刊名：Journal of Biotechnology

收录：EI(收录号：20263421358345);Scopus(收录号：2-s2.0-105048081593)

语种：英文

外文关键词：Biochemistry - Fermentation - Food additives - Glycerol - Metabolic engineering

摘要：Erythritol is a natural zero-calorie sweetener with potential for sustainable healthy diets. While yeasts can convert biodiesel-derived glycerol into erythritol, the low production and by-product accumulation limit its industrial commercialization. Here, we isolated different morphologically Yarrowia lipolytica mutants with higher erythritol production through adaptive laboratory evolution under hyperosmotic stress, in which mutant Z exhibited superior growth performance and membrane-related genetic variants compared to the parent strain. We then systematically engineered strain Z to optimize the flux towards erythritol by improving glycerol utilization, reducing the synthesis of competing sugar alcohols, complementing auxotrophic markers, and boosting precursor supply. After fermentation condition optimization and two-stage fed-batch fermentation in a 5-L bioreactor, the final engineered strain Z12 produced 250.76 g/L erythritol from pure glycerol and 232 g/L erythritol from crude glycerol, which is the highest reported titers for both feedstocks. This study demonstrates the effective integration of adaptive evolution with metabolic remodeling for efficient erythritol biosynthesis. ? 2026 Elsevier B.V.