文献类型：期刊文献

英文题名：Two-stage fractionation of polar alkaloids from Rhizoma coptidis by countercurrent chromatography considering the strategy of reactive extraction

作者：Li, Yang[1];Cai, Fanfan[1];Zhang, Min[2,3];Zhang, Hongyang[1];Wang, Yuerong[1];Hu, Ping[1]

机构：[1]E China Univ Sci & Technol, Sch Chem & Mol Engn, Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab New Drug Design, Shanghai 200237, Peoples R China;[3]E China Univ Sci & Technol, Sch Pharm, Modern Engn Ctr TCM, Shanghai 200237, Peoples R China

年份：2015

卷号：1378

起止页码：58

外文期刊名：JOURNAL OF CHROMATOGRAPHY A

收录：;EI(收录号：20150200413091);WOS:【SCI-EXPANDED(收录号:WOS:000348952500007)】；

基金：This research was financially supported by the National Natural Science Foundation of China (Nos. 81273481 and 21006023), the Fundamental Research Funds from the Central Universities, and National Science and Technology Major Project for Significant New Drugs Development (No. 2013ZX09507005).

语种：英文

外文关键词：Reactive extraction; pH-zone-refining countercurrent chromatography; Rhizoma coptidis; Polar alkaloids; Two-stage fractionation

摘要：Separation of polar alkaloids by countercurrent chromatography (CCC) is challengeable due to their close partition behaviors in solvent system. In this paper, a two-stage method for isolation of epiberberine, jatrorrhizine, palmatine, coptisine, and berberine from Rhizoma coptidis was presented. The first stage separation performed on CCC was based on the principle of reactive extraction. Trifluoroacetic acid was acted as a modulator to selectively react with alkaloids, which changed their partition coefficients in solvent system. Purified epiberberine and other partially separated targets were eluted by ammonium adjusted mobile phase. In the second stage, four alkaloids were purified in pH-zone-refining CCC mode. All the targets collected were over 97% pure determined by HPLC. The method developed demonstrates performing of reactive extraction on standard CCC as an option for separation of polar alkaloids from medicinal plants. (C) 2014 Elsevier B.V. All rights reserved.