文献类型：期刊文献

英文题名：Naphthyridinomycin Biosynthesis Revealing the Use of Leader Peptide to Guide Nonribosomal Peptide Assembly

作者：Pu, Jin-Yue[1];Peng, Chao[1];Tang, Man-Cheng[1];Zhang, Yue[1];Guo, Jian-Ping[1];Song, Li-Qiang[1];Hua, Qiang[2];Tang, Gong-Li[1]

机构：[1]Chinese Acad Sci, State Key Lab Bioorgan & Nat Prod Chem, Shanghai Inst Organ Chem, Shanghai 200032, Peoples R China;[2]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2013

卷号：15

期号：14

起止页码：3674

外文期刊名：ORGANIC LETTERS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000322210600048)】；

基金：We thank Prof. Zixin Deng's laboratory at Shanghai Jiao Tong University for support in obtaining MS data of proteins. Supporting grants include 973 Program 2010CB833200 and 2009CB118901, the National Natural Science Foundation of China (21072214), and the Open Funding Project of the State Key Laboratory of Bioreactor Engineering.

语种：英文

摘要：Analysis of naphthyridinomycin gene cluster revealed that this antibiotic is generated by nonribosomal peptide synthetase (NRPS) machinery. However, four modules encoded by two genes do not correspond with the structural units in the final product. Genetic and biochemical characterization of the gene cluster suggested that the leader peptide mechanism for the NRPS assembly line was Involved in biosynthesis of this tetrahydroisoquinoline alkaloid.