文献类型：期刊文献

中文题名：杂合启动子控制下乙肝病毒表面抗原SA-28融合基因在酵母中的表达

英文题名：The Expression of a Modified HBsAg Gene SA-28 driven by Hybrid Promoter in Yeast

作者：夏杰[1];陆兵[1];徐殿胜[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2000

卷号：26

期号：4

起止页码：354

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：北大核心:【北大核心1996】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：酵母;杂合启动子;基因表达;乙肝病毒表面抗原

外文关键词：Saccharomyces cerevisiae ; hybrid promoter; HBsAg; derepression culture

摘要：在杂合启动子 ADH2 - GAPDH控制下于酿酒酵母中表达乙肝病毒表面抗原 SA- 2 8融合基因的过程研究中 ,确定系统表达的有效阻遏条件为在 1 0 .0 g/ L的葡萄糖浓度下进行细胞的预培养 ,采用稀释操作和碳源饥饿培养等较彻底的去阻遏方式以克服葡萄糖酵解产物阻遏对表达的影响。实验还显示了溶解氧浓度对表达的影响和乙醇流加方式对表达期酵母生长与表达的调节作用。实验获得主培养 4 5h后湿菌体浓度为 1 86 g/ L,细胞内 pre S1效价为 80 0 0 u。
A process for the expression of modified HBsAg gene SA 28 in Saccharomyces cerevisiae dri ven by hybrid promoter ADH2 GAPDH was studied and the conditions for repression and derepression of the expression of gene SA 28 was found out. During the stage of preliminary culture, the glucose concentration for effective repression was about 10.0g/L, and in the stage of expression 24h starvation culture and dilution operation for the expression of gene were carried out. Besides, the effect of oxygen supply and the relationship between growth rate and expression during the phase of induction by means of constant feeding of ethanol are discussed. As the final results, the harvesting wet cell density——186g/L in the fermentation broth and the ELISA units of preS1 antigenicity in the cell——8 000 after 45h main culture were obtained.