文献类型：期刊文献

英文题名：Hyaluronic acid-coated liposome for active targeting on CD44 expressing tumors

作者：Tian, Zhenfen[1,2];Liu, Jianwen[3,4];Li, Na[5];Garamus, Vasil M.[6];Zou, Aihua[1,2]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Chem & Mol Engn, Inst Appl Chem, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, Sch Pharm, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[4]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab New Drug Design, Shanghai 200237, Peoples R China;[5]Shanghai Inst Biochem & Cell Biol, Natl Ctr Prot Sci Shanghai, Shanghai 200237, Peoples R China;[6]Ctr Mat & Coastal Res, Helmholtz Zentrum Geesthacht, D-21502 Geesthacht, Germany

年份：2018

卷号：20

期号：9

外文期刊名：JOURNAL OF NANOPARTICLE RESEARCH

收录：;EI(收录号：20183605780867);WOS:【SCI-EXPANDED(收录号:WOS:000443912800002)】；

基金：This study was funded by National Natural Science Foundation of China (Grant number 21573070). We thank the staff of BL19U2 beamline at National Center for Protein Science Shanghai and Shanghai Synchrotron Radiation Facility (Shanghai, People's Republic of China) for assistance during data collection.

语种：英文

外文关键词：DOX; Liposome; HA; CD44; Target

摘要：Liposome coated with hyaluronic acid (HA) was fabricated for targeted delivery of Doxorubicin hydrochloride (DOX) to CD44 expressing tumors. DOX was incorporated into liposome (DOX-L) via a transmembrane pH-gradient method, which contributed to high encapsulation efficiency (97%) and drug loading (19%). HA was modified on the surface of DOX-L by simple vortex (HA-DOX-L). The average diameter of optimized DOX-L and H-DOX-L was 109.7 +/- 3.1 and 117.2 +/- 5.0 nm, respectively, with good uniformity and stability during 6-month storage. SAXS and TEM evidenced the corona of HA on the surface of DOX-L, which convinced the prolonged circulation of DOX. The apoptosis study demonstrated the improved efficacy of HA-DOX-L with the human colon cancer cell line HCT-116 cells in comparison to the conventional reservoirs. This improved efficacy of HA-DOX-L with HCT-116 cells should be related with the interaction between HA and CD44 receptor of HCT-116 cells.