文献类型：期刊文献

英文题名：Engineering of fast-growing Vibrio natriegens for biosynthesis of poly(3-hydroxybutyrate-co-lactate)

作者：Sun, Xinye[1];Shang, Yanzhe[2];Zhang, Binghao[1];Guo, Pengye[1];Luo, Yuanchan[1];Wu, Hui[1,2,3,4]

机构：[1]East China Univ Sci & Technol, Shanghai Frontiers Sci Ctr Optogenet Tech Cell Met, Sch Biotechnol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Dalian Univ Technol, Sch Bioengn, MOE Key Lab Biointelligent Mfg, Dalian, Peoples R China;[3]Shanghai Collaborat Innovat Ctr Biomfg Technol, 130 Meilong Rd, Shanghai 200237, Peoples R China;[4]Natl Light Ind Council, Key Lab Biobased Mat Engn China, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2024

卷号：11

期号：1

外文期刊名：BIORESOURCES AND BIOPROCESSING

收录：;EI(收录号：20243717021289);WOS:【SCI-EXPANDED(收录号:WOS:001309432400001)】；

基金：This work was supported by the National Natural Science Foundation of China (22278137), the National Key R&D Program of China (2021YFC2103500). Partially supported by Open Funding Project of the State Key Laboratory of Bioreactor Engineering.

语种：英文

外文关键词：Poly(3-hydroxybutyrate-co-lactate); Vibrio natriegens; Metabolic engineering; Lactate fraction

摘要：Poly(3-hydroxybutyrate-co-lactate) [P(3HB-co-LA)] is a highly promising valuable biodegradable material with good biocompatibility and degradability. Vibrio natriegens, owing to its fast-growth, wide substrate spectrum characteristics, was selected to produce P(3HB-co-LA). Herein, the crucial role of acetyltransferase PN96-18060 for PHB synthesis in V. natriegens was identified. Heterologous pathway of P(3HB-co-LA) was introduced into V. natriegens successfully, in addition, overexpression of the dldh gene led to 1.84 fold enhancement of the lactate content in P(3HB-co-LA). Finally, the production of P(3HB-co-LA) was characterized under different carbon sources. The lactate fraction in P(3HB-co-LA) was increased to 28.3 mol% by the modification, about 1.84 times of that of the control. This is the first successful case of producing the P(3HB-co-LA) in V. natriegens. Collectively, this study showed that V. natriegens is an attractive host organism for producing P(3HB-co-LA) and has great potential to produce other co-polymers.