文献类型：期刊文献

英文题名：Population genetics of Cryptosporidium meleagridis in humans and birds: evidence for cross-species transmission

作者：Wang, Yuanfei[1];Yang, Wenli[2];Cama, Vitaliano[3];Wang, Lin[1];Cabrera, Lilia[4];Ortega, Ynes[5];Bern, Caryn[3];Feng, Yaoyu[1];Gilman, Robert[6];Xiao, Lihua[2]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Sch Resources & Environm Engn, Shanghai 200237, Peoples R China;[2]Ctr Dis Control & Prevent, Div Foodborne Waterborne & Environm Dis, Atlanta, GA 30333 USA;[3]Ctr Dis Control & Prevent, Div Parasit Dis & Malaria, Atlanta, GA 30333 USA;[4]Asociac Benef PRISMA, Lima, Peru;[5]Univ Georgia, Ctr Food Safety, Griffin, GA 30223 USA;[6]Johns Hopkins Univ, Dept Int Hlth, Baltimore, MD 21205 USA

年份：2014

卷号：44

期号：8

起止页码：515

外文期刊名：INTERNATIONAL JOURNAL FOR PARASITOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000339038600003)】；

基金：This work was supported by the National Natural Science Foundation of China (31229005 and 31110103901), National Special Fund for State Key Laboratory of Bioreactor Engineering, China (No. 2060204), National Institutes of Health, USA (5P01AI051976 and 5R21AI059661), USDA-CSREES (2001-51110-11340), and Centers for Disease Control and Prevention, USA. The findings and conclusions in this report are those of the authors and do not necessarily represent the views of the Centers for Disease Control and Prevention.

语种：英文

外文关键词：Cryptosporidium meleagridis; Multilocus sequence typing; Subtypes; Population genetics; Zoonosis

摘要：Population genetic studies have been used to understand the transmission of pathogens in humans and animals, especially the role of zoonotic infections and evolution and dispersal of virulent subtypes. In this study, we analysed the genetic diversity and population structure of Cryptosporidium meleagridis, the only known Cryptosporidium species that infects both avian and mammalian hosts and is responsible for approximately 10% of human cryptosporidiosis in some areas. A total of 62 C meleagridis specimens from children, AIDS patients, and birds in Lima, Peru were characterised by sequence analysis of the ssrRNA gene and five minisatellite, microsatellite and polymorphic markers in chromosome 6, including the 60 kDa glycoprotein (gp60), 47 kDa glycoprotein (CP47), a serine repeat antigen (MSC6-5), retinitis pigmentosa GTPase regulator (RPGR) and thrombospondin protein 8 (TSP8). The multilocus sequence analysis identified concurrent infections with Cryptosporidium hominis in four AIDS patients and three children. Unique subtypes of C meleagridis ranged from eight at the gp60 locus (gene diversity Hd = 0.651), three at the RPGR (Hd = 0.556), three at the MSC6-5 locus (Hd = 0.242), two at TSP8 (Hd = 0.198), to one at CP47 (monomorphic), much lower than that of C hominis in the same area. Intra-genic linkage disequilibrium was strong and complete at all gene loci. Intergenic linkage disequilibrium was highly significant (P < 0.001) for all pairs of polymorphic loci. Two major groups of subtypes were seen, with most subtypes belonging to group 1. Within group 1, there was no clear population segregation, and two of the 14 multilocus subtypes of C meleagridis were found in both AIDS patients and birds. We believe that these results provide the first evidence of a clonal population structure of C. meleagridis and the likely occurrence of cross-species transmission of C. meleagridis between birds and humans. Published by Elsevier Ltd. on behalf of Australian Society for Parasitology Inc.