文献类型：期刊文献

中文题名：棘孢小单孢菌绛红变种细胞壁肽聚糖对庆大霉素吸附机制的初步研究

英文题名：Preliminary study of the mechanism of gentamicin bound by peptidoglycan of the cell wall of Micromonospora echinospore var.

作者：牛文泽[1];胡辉[1];储炬[1];李友荣[1]

机构：[1]华东理工大学生物反应器国家重点实验室,上海200237

年份：2003

卷号：28

期号：2

起止页码：65

中文期刊名：中国抗生素杂志

外文期刊名：Chinese Journal of Antibiotics

收录：CSTPCD;;Scopus;北大核心:【北大核心2000】；CSCD:【CSCD2011_2012】；

基金：国家自然科学基金资助项目 (39870 0 1 8)。

语种：中文

中文关键词：棘孢小单孢菌;绛红变种;细胞壁;肽聚糖;庆大霉素;金属离子

外文关键词：Gentamicin; Cell wall; Peptidoglycan; Binding site; Metal ions; Amine

摘要：提取棘孢小单孢菌绛红变种的细胞壁和肽聚糖。肽聚糖是细胞壁的主要成分 ,吸附的庆大霉素占细胞壁吸附总量的 95 %左右。肽聚糖吸附庆大霉素的最适 pH是 4～ 9,当溶液中没有自由状态的庆大霉素存在时 ,肽聚糖最多能吸附庆大霉素约为 880 0 0u/ g肽聚糖 (干重 )。金属离子和胺类化合物都能够竞争肽聚糖上的庆大霉素吸附位点 ,镁离子和钠离子在庆大霉素浓度分别小于 10 0 0u/ml和大于 2 0 0 0u/ml时解吸附效果最好 ,Tris也具有一定的解吸附作用。
Cell wall (CW) and peptidoglycan (PG) were isolated from Micromonospora echinospora. PG, the major part of cell wall, can bind about 95% of the total GM adsorbed on the cell wall. The optimum pH appeared to be 4～9 for the binding of GM by peptidoglycan. The maximum content of bound GM was ca. 88000u/g of dry weight of peptidoglycan and no any free GM was detected in the solution. Metal ions and amine compete with GM for the binding site. Mg 2+ and Na+ desorbed the binding of GM intensively at GM concentration less than 1000u/ml and more than 2000u/ml, respectively. Tris can also release GM from peptidoglycan to a certain degree.