文献类型：期刊文献

英文题名：Detection and elimination of trace d-lactic acid in lignocellulose biorefining chain: Generation, flow, and impact on chiral lactide synthesis

作者：Guo, Xiaomeng[1];Li, Zhibin[1];He, Niling[1];Zhang, Bin[1];Liu, Xiucai[2];Bao, Jie[1,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai, Peoples R China;[2]Cathay Biotech Inc, Shanghai, Peoples R China;[3]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2024

卷号：121

期号：2

起止页码：670

外文期刊名：BIOTECHNOLOGY AND BIOENGINEERING

收录：;EI(收录号：20234415004719);WOS:【SCI-EXPANDED(收录号:WOS:001097764300001)】；

基金：This research was supported by the National Natural Science Foundation of China (21978083) and the Yangfan Project of Science and Technology Committee of Shanghai Municipality (23YF1409900).

语种：英文

外文关键词：biorefinery chain; chiral lactic acid; D-lactic acid; elimination; fingerprint; lignocellulose

摘要：High chiral purity of lactic acid is a crucial indicator for the synthesis of chiral lactide as the primary intermediate chemical for ring-open polymerization of high molecular weight polylactic acid (PLA). Lignocellulose biomass is the most promising carbohydrate feedstock for commercial production of PLA, but the presence of trace d-lactic acid in the biorefinery chain adversely affects the synthesis and quality of chiral lactide. This study analyzed the fingerprint of trace d-lactic acid in the biorefinery chain and found that the major source of d-lactic acid comes from lignocellulose feedstock. The naturally occurring lactic acid bacteria and water-soluble carbohydrates in lignocellulose feedstock provide the necessary conditions for d-lactic acid generation. Three strategies were proposed to eliminate the generation pathway of d-lactic acid, including reduction of moisture content, conversion of water-soluble carbohydrates to furan aldehydes in pretreatment, and conversion to l-lactic acid by inoculating engineered l-lactic acid bacteria. The natural reduction of lactic acid content in lignocellulose feedstock during storage was observed due to the lactate oxidase-catalyzed oxidation of l- and d-lactic acids. This study provided an important support for the production of cellulosic l-lactic acid with high chiral purity.