文献类型：期刊文献

英文题名：The optimization of centrifugal pump driving horizontal tubular photobioreactor for enhancing astaxanthin production using heterotrophic Haematococcus pluvialis

作者：Fan, Fei[1];Fei, Zhongnan[1];Wan, Minxi[1];Huang, Jianke[2];Wang, Weiliang[1];Bai, Wenmin[3];He, Maolei[3];Li, Yuanguang[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Hohai Univ, Coll Oceanog, Nanjing 210098, Peoples R China;[3]Baoshan Zeyuan Algae Hlth Technol Co Ltd, Baoshan 678208, Peoples R China

年份：2021

卷号：341

起止页码：168

外文期刊名：JOURNAL OF BIOTECHNOLOGY

收录：;EI(收录号：20214211019608);WOS:【SCI-EXPANDED(收录号:WOS:000712544600011)】；

基金：This research was funded by National High Technology Research and Development Program (Grant No. 2015AA020602) , High Level Scientific and Technological Talent Project of Yunnan (Grant No. 2015HD005) and Fundamental Research Funds for the Central Universities (Grant number: B200201001) .

语种：英文

外文关键词：Haematococcus pluvialis; Critical shear stress; Horizontal tubular photobioreactor; Centrifugal pump; Scale-up

摘要：Haematococcus pluvialis is the prime source of natural astaxanthin for commercial exploitation. The large-scale cultivation of H. pluvialis is one of the key technologies for the development of natural astaxanthin industry. So far, horizontal tubular photobioreactor (HTPBR) circulated by a centrifugal pump has been the main PBR for the large-scale cultivation of H. pluvialis. Shear stress is a negative factor in microalgal cultivation at different scales, particularly for large-scale cultivation. To reduce the adverse impact of shear stress, the tolerance of H. pluvialis to the shear stress during the induction stage was first investigated in this study. H. pluvialis aplanospore was not sensitive to stresses between 19.18 and 27.32 Pa, but was resulted in about 30% cell death under shear stress between 27.32 and 63.84 Pa. Accordingly, two centrifugal pumps with different impellers was selected in 400 L HTPBRs to study the outdoor photoinduction for astaxanthin accumulation. The highest astaxanthin productivity and astaxanthin concentration were obtained in HTPBRs using a centrifugal pump equipped with three unshrouded backward-bladed impellers. The HTPBR was then successfully scaled up to 800 L with a similar performance, showing good scalability.