文献类型：期刊文献

英文题名：A Sequential Dual-Lock Strategy for Photoactivatable Chemiluminescent Probes Enabling Bright Duplex Optical Imaging

作者：Zhang, Yutao[1,2];Yan, Chenxu[1,2];Wang, Chao[3];Guo, Zhiqian[1,2];Liu, Xiaogang[3];Zhu, Wei-Hong[1,2]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Feringa Nobel Prize Scientist Joint Res Ctr, Inst Fine Chem,Key Lab Adv Mat, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Chem & Mol Engn, Feringa Nobel Prize Scientist Joint Res Ctr, Inst Fine Chem,Joint Int Res Lab Precis Chem & Mo, Shanghai 200237, Peoples R China;[3]Singapore Univ Technol & Design, Fluorescence Res Grp, Sci & Math Cluster, Singapore 487372, Singapore

年份：2020

卷号：59

期号：23

起止页码：9059

外文期刊名：ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

收录：;EI(收录号：20200708183179);WOS:【SCI-EXPANDED(收录号:WOS:000512806600001)】；

基金：This work was supported by NSFC/China (21788102, 21622602, 21636002, 21421004, 21878087, and 21908060), National Key Research and Development Program (2017YFC090690, 2016YFA0200300), Shanghai Municipal Science and Technology Major Project (Grant 2018SHZDZX03), the Innovation Program of Shanghai Municipal Education Commission, Programme of Introducing Talents of Discipline to Universities (B16017), the Shuguang Program (18SG27), the China Postdoctoral Science Foundation (2019M651417), SUTD and the SUTD-MIT International Design Centre (T1SRCI17126, IDG31800104), the SUTD-ZJU Research Collaboration Grant (SD201802). The authors would like to acknowledge the use of the computing service of SUTD-MIT IDC and National Supercomputing Centre, Singapore.

语种：英文

外文关键词：aggregation-induced emission; chemiluminescent probes; dual-mode bioimaging; fluorescence probes; sequential dual-lock

摘要：Chemiluminescence (CL)-based technologies have revolutionized in vivo monitoring of biomolecules. However, significant technical hurdles have limited the achievement of trigger-controlled, bright, and enriched CL signal. Herein, a dual-lock strategy uses sequence-dependent triggers for bright optical imaging with real-time fluorescent signal and ultra-sensitive CL signal. These probes can obtain an analyte-triggered accumulation of stable pre-chemiluminophore with aggregation-induced emission (AIE), and then the pre-chemiluminophore exhibits a rapid photooxidation process (1,2-dioxetane generation) by TICT-based free-radical addition, thereby achieving an enrichment and bright CL signal. The dual-lock strategy expands the in vivo toolbox for highly accurate analysis and has for the first time allowed access to accurately sense and trace biomolecules with high-resolution, dual-mode of chemo-fluoro-luminescence, and three-dimensional (3D) imaging in living animals.