文献类型：期刊文献

英文题名：Protein encapsulation: a new approach for improving the capability of small-molecule fluorogenic probes

作者：Han, Hai-Hao[1,3];Sedgwick, Adam C.[2,4];Shang, Ying[1];Li, Na[5];Liu, Tingting[3];Li, Bo-Han[3];Yu, Kunqian[3];Zang, Yi[3];Brewster, James T., II[4];Odyniec, Maria L.[2];Weber, Maria[2];Bull, Steven D.[2];Li, Jia[3];Sessler, Jonathan L.[4,6];James, Tony D.[2];He, Xiao-Peng[1];Tian, He[1]

机构：[1]East China Univ Sci & Technol, Key Lab Adv Mat, Joint Int Res Lab Precis Chem & Mol Engn, Feringa Nobel Prize Scientist Joint Res Ctr,Sch C, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Univ Bath, Dept Chem, Bath BA2 7AY, Avon, England;[3]Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Natl Ctr Drug Screening, 189 Guo Shoujing Rd, Shanghai 201203, Peoples R China;[4]Univ Texas Austin, Dept Chem, 105 E 24th St A5300, Austin, TX 78712 USA;[5]Zhanjiang Lab, Natl Facil Prot Sci Shanghai, Shanghai 201210, Peoples R China;[6]Shanghai Univ, Dept Chem, Ctr Supramol Chem & Catalysis, 99 Shang Da Rd, Shanghai 200444, Peoples R China

年份：2020

卷号：11

期号：4

起止页码：1107

外文期刊名：CHEMICAL SCIENCE

收录：;EI(收录号：20200608127865);WOS:【SCI-EXPANDED(收录号:WOS:000510929600019)】；

基金：All animal experiments were carried out under the Guidelines for Care and Use of Laboratory Animals of Shanghai Institute of Materia Medica (SIMM), Chinese Academy of Sciences and approved by the Institutional Animal Care and Use Committee (IACUC) of SIMM (Shanghai, China). RAW 264.7 and HeLa were obtained from ATCC (American Type Culture Collection). The authors thank the National Natural Science Foundation of China (Nos. 21788102, 91853201, 21722801, 81673489, 31871414 and U1832144), the Shanghai Municipal Science and Technology Major Project (No. 2018SHZDZX03), the International Cooperation Program of Shanghai Science and Technology Committee (No. 17520750100), the Youth Innovation Promotion Association of Chinese Academy of Sciences (No. 2017319), the Shanghai Science and Technology Committee (No. 19410712600) and the Fundamental Research Funds for the Central Universities (222201717003) for financial support. TDJ wishes to thank the Royal Society for a Wolfson Research Merit Award. The work in Austin was supported by the National Institutes of Health (RO1 GM103790) and the Robert A. Welch Foundation (F-0018). We would like to thank the EPSRC and the University of Bath for funding for ACS and MLO for studentships. MW would like to thank the EPSRC for funding (i) EP/L016354/1 and CDT in Sustainable Chemical Technologies.

语种：英文

外文关键词：Mammals - Proteins - Fluorescence imaging - Probes - Cell culture

摘要：Herein, we report a protein-based hybridization strategy that exploits the host-guest chemistry of HSA (human serum albumin) to solubilize the otherwise cell impermeable ONOO- fluorescent probe Pinkment-OAc. Formation of a HSA/Pinkment-OAc supramolecular hybrid was confirmed by SAXS and solution-state analyses. This HSA/Pinkment-OAc hybrid provided an enhanced fluorescence response towards ONOO(-)versusPinkment-OAc alone, as determined by in vitro experiments. The HSA/Pinkment-OAc hybrid was also evaluated in RAW 264.7 macrophages and HeLa cancer cell lines, which displayed an enhanced cell permeability enabling the detection of SIN-1 and LPS generated ONOO- and the in vivo imaging of acute inflammation in LPS-treated mice. A remarkable 5.6 fold (RAW 264.7), 8.7-fold (HeLa) and 2.7-fold increased response was seen relative to Pinkment-OAc alone at the cellular level and in vivo, respectively. We anticipate that HSA/fluorescent probe hybrids will soon become ubiquitous and routinely applied to overcome solubility issues associated with hydrophobic fluorescent imaging agents designed to detect disease related biomarkers.